Rehm W S, Carrasquer G, Schwartz M
Am J Physiol. 1986 May;250(5 Pt 1):G639-47. doi: 10.1152/ajpgi.1986.250.5.G639.
The primary purpose of this study was to determine the sites of resistance changes from thiocyanate inhibition of secretion to better evaluate models of the gastric proton pump. The potential difference, resistance, and H+ secretory rate were measured with various tonicities of the secretory fluid. Thiocyanate (SCN) inhibition generally produces a resistance increase, but with nutrient Ba, the resistance is high and SCN decreases it. The latter effect is also seen with cimetidine. In the secreting fundus a hypotonic secretory solution has a small effect on resistance, but in the inhibited fundus the effect is huge, due to increase of resistance of the lumen-tubular cell pathway. With a hypotonic secretory solution, for inhibited fundus (cimetidine or omeprazole) and antrum, SCN does not decrease the resistance of the surface cells and/or the transintercellular pathways with or without Ba. The SCN resistance decrease with Ba is via the tubular cell pathway. With Ba the resistance of the nutrient membrane of the tubular cells decreases after SCN or cimetidine inhibition. The findings further support the concept that under standard conditions the resistance via the lumina and tubular cells is low and that via the surface cells and transintercellular pathways is high.
本研究的主要目的是确定从硫氰酸盐抑制分泌导致的阻力变化位点，以更好地评估胃质子泵模型。使用不同渗透压的分泌液测量电位差、阻力和H⁺分泌速率。硫氰酸盐（SCN）抑制通常会导致阻力增加，但使用营养性钡时，阻力较高，而SCN会使其降低。西咪替丁也有后一种作用。在分泌的胃底，低渗分泌液对阻力影响较小，但在受抑制的胃底，由于腔-管状细胞途径阻力增加，这种影响很大。对于低渗分泌液，在受抑制的胃底（西咪替丁或奥美拉唑）和胃窦中，无论有无钡，SCN都不会降低表面细胞和/或细胞间途径的阻力。SCN与钡一起时导致的阻力降低是通过管状细胞途径。使用钡时，SCN或西咪替丁抑制后，管状细胞营养膜的阻力降低。这些发现进一步支持了以下概念：在标准条件下，通过腔和管状细胞的阻力较低，而通过表面细胞和细胞间途径的阻力较高。
