Howard Hughes Medical Institute, Department of Molecular Biology, and Center for Computational and Integrative Biology , Massachusetts General Hospital , Boston , Massachusetts 02114 , United States.
J Org Chem. 2019 Apr 5;84(7):3754-3761. doi: 10.1021/acs.joc.9b00095. Epub 2019 Mar 15.
The ability of the biopolymers RNA and DNA to store and transfer information is essential to life. Herein, we demonstrate template-directed replication in a set of dimer duplexes that use reversible covalent bonds to form base-pairing interactions. Binary sequence information was encoded as a sequence of aniline and benzaldehyde subunits linked together by a diethynyl benzene backbone. These dimers formed sequence-specific, imine-linked duplexes, which could be separated and used as templates for the synthesis of daughter duplexes with identical sequences.
生物聚合物 RNA 和 DNA 存储和传递信息的能力对生命至关重要。在此，我们展示了一组使用可逆共价键形成碱基配对相互作用的二聚体双链体中的模板指导复制。二进制序列信息被编码为苯胺和苯甲醛亚基的序列，通过二乙炔基苯骨架连接在一起。这些二聚体形成了序列特异性的亚胺连接的双链体，可以将其分离并用作合成具有相同序列的子双链体的模板。
