State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China; Department of Biochemistry and Molecular Biology, School of Basic Medicine and the Collaborative Innovation Center for Brain Science, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China.
J Mech Behav Biomed Mater. 2019 Jun;94:42-50. doi: 10.1016/j.jmbbm.2019.02.026. Epub 2019 Feb 25.
In recent years, the magnesium phosphate cements showed impressive advantages for their setting property, mechanical strength, and resorption rate in laboratory investigation. While it remained a big challenge to develop the magnesium phosphate cements with ideal self-setting properties, sufficient mechanical strength, excellent biocompatibility, and osteoinductivity for clinical application. In our work, we prepared the magnesium calcium phosphate cement (MCPC) using the MgO, KHPO, and Ca(HPO) particles with the citric acid added. The citric acid was adopted to modify the setting time and compressive strength of the MCPC, which were investigated by the X-ray diffractometer and scanning electron microscopy. The cytocompatibility and osteoinductivity of the modified cements were evaluated by the MC3T3-E1 cells proliferation and morphology, alkaline phosphatase assay, alizarin red staining and western blot assay. The results demonstrated that the citric acid modified MCPC was featured of satisfactory setting time, ideal mechanical strength, good cytocompatibility and osteoinductivity, indicating its potential application for bone regeneration.
近年来，磷酸镁水泥因其凝固性能、力学强度和体内吸收速率在实验室研究中表现出显著优势。然而，开发具有理想自凝固性能、足够力学强度、优异生物相容性和骨诱导性的磷酸镁水泥仍然是一个巨大的挑战，难以满足临床应用的需要。在我们的工作中，我们使用 MgO、KHPO 和 Ca(HPO)颗粒，并添加柠檬酸来制备镁钙磷酸盐水泥（MCPC）。采用柠檬酸来调节 MCPC 的凝固时间和抗压强度，并通过 X 射线衍射仪和扫描电子显微镜进行了研究。通过 MC3T3-E1 细胞增殖和形态、碱性磷酸酶测定、茜素红染色和 Western blot 分析来评估改性水泥的细胞相容性和骨诱导性。结果表明，柠檬酸改性的 MCPC 具有令人满意的凝固时间、理想的力学强度、良好的细胞相容性和骨诱导性，表明其在骨再生方面具有潜在的应用前景。
