Lanzhou University Second Hospital, Lanzhou, 730030, P. R. China.
School of Pharmacy, Lanzhou University, Lanzhou, 730000, P. R. China.
J Mater Sci Mater Med. 2024 Aug 13;35(1):49. doi: 10.1007/s10856-024-06818-7.
It's imperative to create a more ideal biological scaffold for bone defect repair. Calcium phosphate bone cements (CPC) could be used as a scaffold. Some ingredients and osteogenic factors could be added to improve its poor mechanical properties and biological activity. As a macromolecule extracted from traditional Chinese medicine, Hedysarum polysaccharides (HPS) would significantly promote the osteogenic activity of bone biomaterials. Zirconium oxide and starch were added to the solid phase and citric acid was added to the liquid phase to optimize CPC. HPS was loaded onto the scaffold as an osteogenic factor, and the prepared CPS + HPS was characterized. Further, the cytocompatibility of CPS + HPS was assessed according to activity, differentiation, and calcification in neonatal rat calvarial osteoblasts, and the biosafety of CPS + HPS was evaluated according to acute toxicity, pyrogen, sensitization, and hemolysis. The success of CPS + HPS in repairing bone defects was evaluated by using a rabbit femur implantation experiment. After optimization, CPS-20-CA-5 containing 10% starch and 5% citric acid displayed the highest mechanical strength of 28.96 ± 0.03 MPa. HPS-50 was demonstrated to exert the best osteogenic effect. The combination of CPS + HPS achieved HPS-loaded CPC. Material characterization, cytocompatibility, biosafety, and femoral implantation experiments indicated that CPS + HPS possessed better pressure resistance and improved osteogenic ability in bone defect repair.CPS + HPS demonstrated effective pressure resistance and superior osteogenic ability, which may be of great significance for bone defects and bone tissue engineering to promote bone regeneration and repair.
必须为骨缺损修复创造更理想的生物支架。磷酸钙骨水泥（CPC）可用作支架。可以添加一些成分和成骨因子来改善其较差的机械性能和生物活性。作为一种从中药中提取的大分子，黄芪多糖（HPS）可显著促进骨生物材料的成骨活性。将氧化锆和淀粉添加到固相，柠檬酸添加到液相以优化 CPC。将 HPS 加载到支架上作为成骨因子，对制备的 CPS+HPS 进行了表征。进一步，根据活性、分化和新生大鼠颅骨成骨细胞的钙化评估 CPS+HPS 的细胞相容性，并根据急性毒性、热原、致敏和溶血评估 CPS+HPS 的生物安全性。通过兔股骨植入实验评估 CPS+HPS 修复骨缺损的效果。优化后，含有 10%淀粉和 5%柠檬酸的 CPS-20-CA-5 显示出最高的机械强度 28.96±0.03 MPa。HPS-50 表现出最佳的成骨效果。CPS+HPS 的组合实现了 HPS 负载的 CPC。材料特性、细胞相容性、生物安全性和股骨植入实验表明，CPS+HPS 具有更好的抗压能力和改善骨缺损修复中的成骨能力。
CPS+HPS 表现出有效的抗压能力和优异的成骨能力，这对于骨缺损和骨组织工程促进骨再生和修复可能具有重要意义。
