Lyu W L, Deng W, Liu D Y, Yun X Y, Li C Y
Department of General Dentistry, Stomatological Hospital, Tianjin Medical University, Tianjin 300070, China.
Department of Stomatology, The First Affiliated Hospital of Gannan Medical University, Ganzhou 341000, China.
Zhonghua Kou Qiang Yi Xue Za Zhi. 2019 Mar 9;54(3):183-187. doi: 10.3760/cma.j.issn.1002-0098.2019.03.007.
To investigate the effects of two nanotopographies of ultraviolet (UV)-treated titanium surface on macrophage biological behaviour and inflammatory cytokines secretion, and to provide basis for clinical application of UV-treatment in dental implant modification. Titanium disks were allocated into two groups. Samples in one group were acid-etched in hydrofluoric acid (Acid Ti group), and those in the other group were acid-etched and anodized (Anodization group) to form two nanotopographies respectively. The surface morphology was evaluated by field-emission scanning electron microscopy (FE-SEM). The samples were stored in the dark for 8 weeks. Thirteen samples from each group were exposed to UV-irradiation for 48 h (Acid Ti+UV group and Anodization+UV group), UV-untreated samples from Acid Ti and Anodization groups served as control. Hydrophilicity of samples was measured using contact angle measuring device. After 4, 24 and 72 h of incubation, macrophage cell adhesion and proliferation were conducted using cell counting kit-8. Cytokine/chemokine secretions [tumor necrosis factor-α (TNF-α), monocyte chemotactic protein-1 (MCP-1) and macrophage inflammatory protein-1α (MIP-1α)] were measured from cell culture supernatants at 24 and 72 h using magnetic luminex assay. Cell morphology was examined using FE-SEM after 2 h of incubation. Micropitted/nanopillar and micropitted/nanotubular topographies were observed in Acid Ti group and Anodization group respectively. Contact angles in Acid Ti+UV and Anodization+UV groups (20.2°±2.8° and 0.0°±0.0°) were significantly smaller than those in the Acid Ti and Anodization groups (0.05). Cell adhesion and proliferation in all groups at 4 and 24 h showed no difference (0.05). Cell proliferation in Acid Ti+UV and Anodization+UV groups at 72 h were (0.92±0.13) and (1.10±0.08) respectively, which were significantly higher than those in Acid Ti and Anodization groups. TNF-α concentration in Acid Ti+UV and Anodization+UV groups at 72 h were (1.03±0.11) and (0.87±0.10) ng/L, MCP-1 were (301.7±50.3) and (240.8±18.7) ng/L, MIP-1α were (224.9±30.6) and (233.9±14.9) ng/L respectively, which were significantly lower than those in Acid Ti and Anodization groups (0.05). UV treatment can increase hydrophilicity of two titanium surface topographies, especially of Anodization+UV group. UV-treated titanium surfaces can promote macrophage proliferation and reduce the inflammatory response .
为研究紫外线（UV）处理的钛表面两种纳米拓扑结构对巨噬细胞生物学行为及炎性细胞因子分泌的影响，为UV处理在牙种植体改性临床应用中提供依据。将钛盘分为两组。一组样品在氢氟酸中进行酸蚀（酸蚀钛组），另一组样品先酸蚀再进行阳极氧化（阳极氧化组），分别形成两种纳米拓扑结构。通过场发射扫描电子显微镜（FE-SEM）评估表面形貌。样品在黑暗中保存8周。每组13个样品接受UV照射48小时（酸蚀钛+UV组和阳极氧化+UV组），酸蚀钛组和阳极氧化组未经过UV处理的样品作为对照。使用接触角测量仪测量样品的亲水性。孵育4、24和72小时后，使用细胞计数试剂盒-8检测巨噬细胞的黏附和增殖情况。在24和72小时时，使用磁珠免疫分析从细胞培养上清液中检测细胞因子/趋化因子[肿瘤坏死因子-α（TNF-α）、单核细胞趋化蛋白-1（MCP-1）和巨噬细胞炎性蛋白-1α（MIP-1α）]的分泌。孵育2小时后，使用FE-SEM检查细胞形态。酸蚀钛组和阳极氧化组分别观察到微坑/纳米柱和微坑/纳米管拓扑结构。酸蚀钛+UV组和阳极氧化+UV组的接触角（20.2°±2.8°和0.0°±0.0°）显著小于酸蚀钛组和阳极氧化组（P<0.05）。所有组在4和24小时时的细胞黏附和增殖无差异（P>0.05）。酸蚀钛+UV组和阳极氧化+UV组在72小时时的细胞增殖分别为（0.92±0.13）和（1.10±0.08），显著高于酸蚀钛组和阳极氧化组。酸蚀钛+UV组和阳极氧化+UV组在72小时时的TNF-α浓度分别为（1.03±0.11）和（0.87±0.10）ng/L，MCP-1分别为（301.7±50.3）和（240.8±18.7）ng/L，MIP-1α分别为（224.9±30.6）和（233.9±14.9）ng/L，均显著低于酸蚀钛组和阳极氧化组（P<0.05）。UV处理可增加两种钛表面拓扑结构的亲水性，尤其是阳极氧化+UV组。UV处理的钛表面可促进巨噬细胞增殖并减轻炎症反应。
