Forbes Gregory A, Goodwin Stephen B, Drenth André, Oyarzun Pedro, Ordoñez Maria Eugenia, Fry William E
International Potato Center (CIP).
USDA-ARS, Department of Botany and Plant Pathology, 1155 Lilly Hall, Purdue University, West Lafayette, IN 47907.
Plant Dis. 1998 Jul;82(7):811-818. doi: 10.1094/PDIS.1998.82.7.811.
A marker database was compiled for isolates of the potato and tomato late blight pathogen, Phytophthora infestans, originating from 41 locations which include 31 countries plus 10 regions within Mexico. Presently, the database contains information on 1,776 isolates for one or more of the following markers: restriction fragment length polymorphism (RFLP) "fingerprint" consisting of 23 bands; mating type; dilocus allozyme genotype; mitochondrial DNA haplotype; sensitivity to the fungicide metalaxyl; and virulence. In the database, 305 entries have unique RFLP fingerprints and 258 entries have unique multilocus genotypes based on RFLP fingerprint, dilocus allozyme genotype, and mating type. A nomenclature is described for naming multilocus genotypes based on the International Organization for Standardization (ISO) two-letter country code and a unique number. Forty-two previously published multilocus genotypes are represented in the database with references to publications. As a result of compilation of the database, seven new genotypes were identified and named. Cluster analysis of genotypes from clonally propagated populations worldwide generally confirmed a previously published classification of "old" and "new" genotypes. Genotypes from geographically distant countries were frequently clustered, and several old and new genotypes were found in two or more distant countries. The cluster analysis also demonstrated that A2 genotypes from Argentina differed from all others. The database is available via the Internet, and thus can serve as a resource for Phytophthora workers worldwide.
为马铃薯和番茄晚疫病病原菌致病疫霉（Phytophthora infestans）的分离株建立了一个标记数据库，这些分离株来自41个地点，包括31个国家以及墨西哥境内的10个地区。目前，该数据库包含1776个分离株的以下一种或多种标记信息：由23条带组成的限制性片段长度多态性（RFLP）“指纹图谱”；交配型；双基因座等位酶基因型；线粒体DNA单倍型；对杀菌剂甲霜灵的敏感性；以及毒力。在数据库中，305个条目具有独特的RFLP指纹图谱，258个条目基于RFLP指纹图谱、双基因座等位酶基因型和交配型具有独特的多位点基因型。描述了一种基于国际标准化组织（ISO）双字母国家代码和唯一编号来命名多位点基因型的命名法。数据库中列出了42个先前发表的多位点基因型，并给出了相关出版物的参考文献。由于数据库的汇编，鉴定并命名了7个新基因型。对全球克隆繁殖群体的基因型进行聚类分析，总体上证实了先前发表的“旧”基因型和“新”基因型分类。来自地理上遥远国家的基因型经常聚类在一起，在两个或更多遥远国家发现了几种旧基因型和新基因型。聚类分析还表明，来自阿根廷的A2基因型与所有其他基因型不同。该数据库可通过互联网获取，因此可为全球致病疫霉研究人员提供资源。
