Byrne J M, Hausbeck M K, Meloche C, Jarosz A M
Graduate Assistant.
Assistant Professor.
Plant Dis. 1998 Jun;82(6):639-641. doi: 10.1094/PDIS.1998.82.6.639.
Effects of temperature, duration of leaf wetness, and leaf position on foliar infection of greenhouse-grown tomato (cv. Bonnie Best) by Colletotrichum coccodes were determined by inoculating plants with C. coccodes (5.0 × 10 conidia per ml) and keeping them in a dew chamber for 0, 4, 8, 12, 16, 20, or 24 h of wetting at 15, 20, or 25°C. One week after inoculation, leaf disks were placed on the surface of an amended medium on which colonies of C. coccodes were compact and easily identified, and severity of infection was quantified after 4, 6, and 8 days. There was no infection of plants incubated at 15°C, while plants kept at 20 or 25°C had increasing numbers of colonies when leaf wetness duration was extended beyond 12 and 8 h, respectively. Leaf position had a significant effect, with leaves tending to increase in susceptibility as they age. After 24 h of leaf wetness at 25°C, the mean number of colonies per leaf disk from top, middle, and bottom leaves was 23.8, 29.0, and 34.0, respectively.
通过用椰色刺盘孢（每毫升5.0×10个分生孢子）接种温室种植的番茄（品种Bonnie Best）植株，并将其置于露室中，在15、20或25℃下保持叶片湿润0、4、8、12、16、20或24小时，测定了温度、叶片湿润持续时间和叶位对椰色刺盘孢引起的温室番茄叶部感染的影响。接种一周后，将叶盘放置在改良培养基表面，椰色刺盘孢的菌落紧密且易于识别，在4、6和8天后对感染严重程度进行量化。在15℃下培养的植株未被感染，而分别在20或25℃下培养的植株，当叶片湿润持续时间分别超过12小时和8小时时，菌落数量增加。叶位有显著影响，随着叶片变老，其易感性往往增加。在25℃下叶片湿润24小时后，顶部、中部和底部叶片每个叶盘的平均菌落数分别为23.8、29.0和34.0。
