Institute of Medicine, Chung Shan Medical University, Taichung 40201, Taiwan.
Women's Health Research Laboratory, Changhua Christian Hospital, Changhua 50006, Taiwan.
Int J Environ Res Public Health. 2019 Mar 8;16(5):841. doi: 10.3390/ijerph16050841.
The aim of this study was to examine the effects of single-nucleotide polymorphisms (SNPs) in the anti-Müllerian hormone (AMH) and AMH type II receptor (AMHRII) genes on in vitro fertilization (IVF) outcomes. In this prospective cohort study, we genotyped the AMH 146 T > G, AMHRII -482 A > G and AMHRII IVS1 +149 T > A variants in 635 women undergoing their first cycle of controlled ovarian stimulation for IVF. DNA was extracted from the peripheral blood of all participants, and the SNPs were genotyped by real-time polymerase chain reaction. The distributions, frequencies of genes, and correlation with clinical pregnancy of IVF were analyzed. The AMH 146 T > G G/G genotype in women was associated with a lower clinical pregnancy rate (T/T: 55.0%, T/G: 51.8%, G/G: 40.0%; < 0.05). Women with the AMH 146 T > G GG genotype were half as likely to have a clinical pregnancy compared with women with TT genotypes (OR = 0.55, 95% CI: 0.34⁻0.88, = 0.014). With multivariate analysis, the AMH 146 T > G GG genotype remains as a significant independent factor to predict clinical pregnancy ( = 0.014). No significant difference was found between AMHRII polymorphisms and clinical pregnancy outcomes of IVF. In conclusion, our results show that AMH 146 T > G seems to be a susceptibility biomarker capable of predicting IVF pregnancy outcomes. Further studies should focus on the mechanism of these associations and the inclusion of other ethnic populations to confirm the findings of this study.
本研究旨在探讨抗苗勒管激素（AMH）和 AMH 型 II 受体（AMHRII）基因中的单核苷酸多态性（SNPs）对体外受精（IVF）结局的影响。在这项前瞻性队列研究中，我们对 635 名接受控制性卵巢刺激以进行 IVF 的女性的 AMH 146 T > G、AMHRII -482 A > G 和 AMHRII IVS1 +149 T > A 变体进行了基因分型。从所有参与者的外周血中提取 DNA，并通过实时聚合酶链反应对 SNPs 进行基因分型。分析了 IVF 的 SNP 分布、基因频率及其与临床妊娠的相关性。女性 AMH 146 T > G G/G 基因型与较低的临床妊娠率相关（T/T：55.0%，T/G：51.8%，G/G：40.0%；<0.05）。与 TT 基因型相比，携带 AMH 146 T > G GG 基因型的女性发生临床妊娠的可能性降低一半（OR=0.55，95%CI：0.34⁻0.88， = 0.014）。多变量分析显示，AMH 146 T > G GG 基因型仍然是预测临床妊娠的独立显著因素（ = 0.014）。AMHRII 多态性与 IVF 临床妊娠结局之间未发现显著差异。总之，我们的研究结果表明 AMH 146 T > G 似乎是一种能够预测 IVF 妊娠结局的易感生物标志物。进一步的研究应关注这些关联的机制，并纳入其他种族群体以验证本研究的结果。
