Tsyrlov I B, Chasovnikova O B, Grishanova A Iu, Liakhovich V V
Biokhimiia. 1986 Apr;51(4):579-89.
Injection of Wistar rats with five structurally different inducers of methylcholanthrene-type (polycyclic aromatic and heterocyclic hydrocarbons, chloro-derivatives of biphenyl and dibenzo-p-dioxin) results in the de novo synthesis of two P-448 hemoproteins (molecular weight 56 000 and 53 000 Da) differing in their functional and immunochemical properties in liver microsomes. A comparison of catalytic and immunochemical characteristics of five cytochrome P-448 forms (Mr = 56 000 Da) as well as the data from electrophoretic, proteolytic and inhibitory analyses revealed no differences in the preparations used, with the exception of 2,3,7,8-tetrachloro-dibenzo-p-dioxin-induced microsomes characterized by a low level of this cytochrome P-448 form and a higher molecular activity as compared with 3,4-benzpyrene and 7-ethoxyresorufin-induced microsomes. The experimental results do not confirm the hypothesis on the feasibility of induced synthesis of a variety of individual forms of monooxygenase that would correlate with the number of structural variants of inducers.
给Wistar大鼠注射五种结构不同的甲基胆蒽型诱导剂（多环芳烃和杂环烃、联苯氯衍生物和二苯并 - p - 二噁英），会导致肝脏微粒体中从头合成两种P - 448血红素蛋白（分子量分别为56000和53000 Da），它们在功能和免疫化学性质上存在差异。对五种细胞色素P - 448形式（Mr = 56000 Da）的催化和免疫化学特性进行比较，以及电泳、蛋白水解和抑制分析的数据显示，除了2,3,7,8 - 四氯二苯并 - p - 二噁英诱导的微粒体外，所用制剂之间没有差异，与3,4 - 苯并芘和7 - 乙氧基试卤灵诱导的微粒体相比，该微粒体中这种细胞色素P - 448形式的水平较低，但分子活性较高。实验结果并未证实关于诱导合成多种与诱导剂结构变体数量相关的单加氧酶个体形式的可行性这一假设。
