Duzhak T G, Tsyrlov I B
Biokhimiia. 1987 Feb;52(2):214-9.
Two cytochrome P-448 fractions, B1 and B2, were isolated from liver microsomes of 3,4-benzpyrene-induced inbred C57Bl/6 mice, using chromatography on octyl-Sepharose CL-4B and on Whatman 52E. During subsequent chromatography on hydroxylapatite fraction B1 was separated into 2 subfractions, G1 and G2. Cytochrome fractions B1, G1 and G2 have similar "peptide maps" differing from that of fraction B2. Cytochrome fraction B1 is immunologically identical to G2, partly to fraction B2 but is distinct from fraction G1. Fraction G2 is identified as the form of cytochrome P-448 catalyzing the hydroxylation of 3,4-benzpyrene and 7-ethoxyresorufin and existing in a low spin form. Cytochrome fraction G1 is apparently identical to the form P3-450. Fraction B2 was not yet described in current literature, since cytochrome P-448 (Mr = 53,000 Da) was identified only after the induction of mice with 3,4-benzpyrene but not with other inducers, e.g., polycyclic aromatic hydrocarbons.
利用辛基-琼脂糖凝胶CL-4B柱色谱法和沃特曼52E滤纸色谱法，从经3,4-苯并芘诱导的近交系C57Bl/6小鼠肝脏微粒体中分离出两种细胞色素P-448组分，即B1和B2。在随后的羟基磷灰石柱色谱过程中，B1组分被分离成两个亚组分，即G1和G2。细胞色素组分B1、G1和G2具有相似的“肽图”，与B2组分的“肽图”不同。细胞色素组分B1在免疫学上与G2相同，部分与B2组分相同，但与G1组分不同。G2组分被鉴定为催化3,4-苯并芘和7-乙氧基试卤灵羟基化反应的细胞色素P-448形式，且以低自旋形式存在。细胞色素组分G1显然与P3-450形式相同。目前的文献中尚未描述B2组分，因为细胞色素P-448（分子量=53,000道尔顿）仅在经3,4-苯并芘诱导的小鼠中被鉴定出来，而在经其他诱导剂（如多环芳烃）诱导的小鼠中未被鉴定出来。
