Cash R, Raisman R, Lanfumey L, Ploska A, Agid Y
Brain Res. 1986 Apr 2;370(1):127-35. doi: 10.1016/0006-8993(86)91112-1.
The localization of adrenergic receptors in the central nervous system was studied in two physiological conditions of noradrenergic denervation, a 6-hydroxydopamine-induced lesion of the locus coeruleus in newborn rat, and a pathological related degeneration of the locus coeruleus in man, Parkinson's disease. The localization of these receptors in the synapse has been studied with the technique of subcellular fractionation by differential centrifugation. In lesioned rats, an increase in the density of alpha 1 and beta 1 receptors was observed in several brain regions, in contrast to alpha 2 receptors which were not modified. Subcellular fractionation in lesioned rats showed an increase in alpha 1 and beta 1 receptors in synaptosomal fractions. Similar results were found in parkinsonian patients: alpha 1 receptors increased in the synaptosomal fraction; beta receptors increased in synaptosomal and microsomal fractions. These results suggest that alpha 1 and beta 1 receptors may be located on non-noradrenergic nerve terminals in mammalian brain. alpha 2 and beta 2 receptors may be situated on glial cells or neuronal elements unrelated to noradrenergic input.
在两种去甲肾上腺素能失神经支配的生理条件下，研究了中枢神经系统中肾上腺素能受体的定位，这两种条件分别是新生大鼠中6-羟基多巴胺诱导的蓝斑损伤，以及人类帕金森病中与病理相关的蓝斑变性。通过差速离心进行亚细胞分级分离技术，研究了这些受体在突触中的定位。在受损大鼠中，在几个脑区观察到α1和β1受体密度增加，而α2受体未发生改变。受损大鼠的亚细胞分级分离显示，突触体部分的α1和β1受体增加。在帕金森病患者中也发现了类似结果：突触体部分的α1受体增加；突触体和微粒体部分的β受体增加。这些结果表明，α1和β1受体可能位于哺乳动物脑内的非去甲肾上腺素能神经末梢上。α2和β2受体可能位于与去甲肾上腺素能输入无关的神经胶质细胞或神经元成分上。
