用于检测临床样本中疟原虫的电化学纳米传感器的研发。
Development of Electrochemical Nanosensor for the Detection of Malaria Parasite in Clinical Samples.
作者信息
Obisesan Olaoluwa R, Adekunle Abolanle S, Oyekunle John A O, Sabu Thomas, Nkambule Thabo T I, Mamba Bhekie B
机构信息
Department of Chemistry, Obafemi Awolowo University, Ile-Ife, Nigeria.
International and Inter University Center for Nanoscience and Nanotechnology, Mahatma Gandhi University, Kottayam, India.
出版信息
Front Chem. 2019 Feb 25;7:89. doi: 10.3389/fchem.2019.00089. eCollection 2019.
In this study, electrochemical nanosensors were developed from the synthesized metal oxide (MO) nanoparticles by supporting it on a gold electrode (Au). The activity of the developed nanosensor toward the detection of malaria biomarker (β-hematin) was determined and the optimum conditions at which the maximum detection and quantification occurred were established. β-Hematin current response at the sensors was higher when compared with the bare Au electrode and followed the order Au-CuO (C) > Au-CuO (M) > Au-FeO3 (M) > Au-FeO3 (C) > Au-AlO3 (M) > Au-AlO3 (C) > bare Au. The developed sensors were stable with a relatively low current drop (10.61-17.35 %) in the analyte. Au-CuO sensor had the best performance toward the biomarker and quantitatively detected in infected mice's serum samples at 3.60-4.8 mM and in human blood serum samples at 0.65-1.35 mM concentration.
在本研究中，通过将合成的金属氧化物（MO）纳米颗粒负载在金电极（Au）上，开发了电化学纳米传感器。测定了所开发的纳米传感器对疟疾生物标志物（β-血红素）的检测活性，并确定了实现最大检测和定量的最佳条件。与裸金电极相比，传感器上的β-血红素电流响应更高，顺序为Au-CuO（C）>Au-CuO（M）>Au-FeO3（M）>Au-FeO3（C）>Au-AlO3（M）>Au-AlO3（C）>裸Au。所开发的传感器具有稳定性，在分析物中的电流下降相对较低（10.61-17.35%）。Au-CuO传感器对生物标志物具有最佳性能，能够在感染小鼠血清样本中以3.60-4.8 mM的浓度以及在人血清样本中以0.65-1.35 mM的浓度进行定量检测。
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