Miguez C B, Beveridge T J, Ingram J M
Can J Microbiol. 1986 Mar;32(3):248-53. doi: 10.1139/m86-049.
Pseudomonas aeruginosa ATCC 9027 grew on 0.5% (v/v) hexadecane as a sole carbon source in a chemically defined medium which required the addition of Fe3+ and Ca2+. There was a variable and extended lag period before an active growth rate was attained. Visible light microscopic evidence revealed that the bacteria did not adhere to hexadecane droplets suggesting the absence of a bioemulsifier. When compared with glucose-grown cells, hexadecane-grown cells produced 75% less lipopolysaccharide (on a total protein basis); this lipopolysaccharide contained 30-40% less carbohydrate, yet 50-75% more 2-keto-3-deoxyoctonate. These chemical changes made the cell surface appear more hydrophobic when tested in a biphasic hydrophobicity index system. Electron microscopy of thin sections and freeze etchings revealed hexadecane-grown cells contained granules which were judged to be polyphosphate by energy dispersive X-ray analysis. There was no apparent major morphological envelope alteration within the two cell types.
铜绿假单胞菌ATCC 9027在一种化学限定培养基中，以0.5%（v/v）十六烷作为唯一碳源生长，该培养基需要添加Fe3+和Ca2+。在达到活跃生长速率之前，存在一个可变的且延长的延迟期。可见光显微镜证据显示，细菌不附着于十六烷液滴，这表明不存在生物乳化剂。与以葡萄糖生长的细胞相比，以十六烷生长的细胞产生的脂多糖减少了75%（基于总蛋白）；这种脂多糖含有的碳水化合物减少了30 - 40%，但2-酮-3-脱氧辛酸增加了50 - 75%。在双相疏水性指数系统中进行测试时，这些化学变化使细胞表面显得更疏水。超薄切片和冷冻蚀刻的电子显微镜观察显示，以十六烷生长的细胞含有颗粒，通过能量色散X射线分析判断这些颗粒为多聚磷酸盐。两种细胞类型之间没有明显的主要形态包膜改变。
