Laboratory of 3-D structures of microorganisms, G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Prospect Nauki, 5 Pushchino, Moscow Region, 142290 Russian Federation.
Department of Environmental Sciences, University of California, 900 University Ave, Riverside, CA 92521, USA.
FEMS Yeast Res. 2017 May 1;17(3). doi: 10.1093/femsyr/fox026.
Canals are supramolecular complexes observed in the cell wall of Candida maltosa grown in the presence of hexadecane as a sole carbon source. Such structures were not observed in glucose-grown cells. Microscopic observations of cells stained with diaminobenzidine revealed the presence of oxidative enzymes in the canals. 4΄,6΄-diamino-2-phenylindole staining revealed that a substantial part of cellular polyphosphate was present in the cell wall of cells grown on hexadecane in condition of phosphate limitation. The content and chain length of polyphosphates were higher in hexadecane-grown cells than in glucose grown ones. The treatment of cells with yeast polyphosphatase PPX1 resulted in the decrease of the canal size. These data clearly indicated that polyphosphates are constituents of canals; they might play an important role in the canal structure and functioning.
在以十六烷作为唯一碳源培养的产朊假丝酵母细胞壁中观察到了管状超分子复合物。在以葡萄糖作为碳源培养的细胞中未观察到这种结构。用二氨基联苯胺对细胞进行染色的显微镜观察显示，管状结构中存在氧化酶。4΄,6΄-二氨基-2-苯基吲哚染色显示，在磷酸盐限制条件下，以十六烷作为碳源培养的细胞中，相当一部分细胞多聚磷酸盐存在于细胞壁中。在十六烷培养的细胞中，多聚磷酸盐的含量和链长均高于在葡萄糖培养的细胞中。用酵母多聚磷酸酶 PPX1 处理细胞会导致管状结构变小。这些数据清楚地表明，多聚磷酸盐是管状结构的组成部分；它们可能在管状结构的形成和功能中发挥重要作用。
