Salkinoja-Salonen M, Nurmiaho E L
J Gen Microbiol. 1978 Mar;105(1):23-8. doi: 10.1099/00221287-105-1-23.
The surface structure of Pseudomonas aeruginosa PACl and PAClR and of lipopolysaccharide-defective mutants derived from them was studied by negative-staining and thin-section electron microscopy and compared with that of a rough mutant with wild-type lipopolysaccharide. The rough mutant and the parent strains had fairly smooth outer layers. Negatively stained preparations of all the mutants lacking polymerized O-antigenic sidechains, including a semi-rough mutant, showed numerous blebs on the surface. In thin sections of these mutants occasional extrusions from the surface were seen. They appeared to consist of material extruded from the outer membrane, but there was no evidence to suggest they were complete unit membranes. Polymerized O-antigenic side-chains in the lipopolysaccharide appear to be required to produce the wild-type appearance of the outer membrane in P. aeruginosa.
通过负染色和超薄切片电子显微镜研究了铜绿假单胞菌PACl和PAClR及其衍生的脂多糖缺陷型突变体的表面结构，并与具有野生型脂多糖的粗糙型突变体进行了比较。粗糙型突变体和亲本菌株具有相当光滑的外层。所有缺乏聚合O抗原侧链的突变体（包括一个半粗糙型突变体）的负染色制剂在表面显示出大量泡状突起。在这些突变体的超薄切片中偶尔可见表面有突出物。它们似乎由从外膜挤出的物质组成，但没有证据表明它们是完整的单位膜。脂多糖中的聚合O抗原侧链似乎是铜绿假单胞菌外膜呈现野生型外观所必需的。
