Mitchell R L, Henning-Chubb C, Huberman E, Verma I M
Cell. 1986 May 23;45(4):497-504. doi: 10.1016/0092-8674(86)90281-3.
The c-fos gene is rapidly and transiently expressed when human U-937 and HL-60 leukemia cells are induced to differentiate to macrophages. We show that the expression of c-fos is controlled primarily at the transcriptional level. c-fos mRNA is very labile, with a half-life of less than 30 min. Superinduction of c-fos in the presence of cycloheximide occurs primarily because of stabilization of c-fos mRNA. When U-937 cells are serum-stimulated or treated with diacylglycerol, a c-kinase agonist, c-fos is transiently expressed to high levels; however, the cells fail to differentiate to macrophages. Furthermore, HL-60 cell variants resistant to TPA can be induced to differentiate to macrophages in the absence of detectable c-fos expression.
当人U - 937和HL - 60白血病细胞被诱导分化为巨噬细胞时，c - fos基因会快速且短暂地表达。我们发现c - fos的表达主要在转录水平受到调控。c - fos mRNA非常不稳定，半衰期不到30分钟。在环己酰亚胺存在的情况下c - fos的超诱导主要是由于c - fos mRNA的稳定化。当U - 937细胞受到血清刺激或用二酰基甘油（一种蛋白激酶C激动剂）处理时，c - fos会短暂地高水平表达；然而，这些细胞无法分化为巨噬细胞。此外，对佛波酯（TPA）耐药的HL - 60细胞变体在没有可检测到的c - fos表达的情况下也能被诱导分化为巨噬细胞。
