Calabretta B
Mol Cell Biol. 1987 Feb;7(2):769-74. doi: 10.1128/mcb.7.2.769-774.1987.
Treatment of five human myeloid leukemic cell lines (KG1, ML3, HL-60, U-937, and HEL) with TPA was followed by macrophage differentiation and was accompanied by an early and transient increase in the mRNA level of c-fos proto-oncogene. The induction of c-fos was also observed in human cell lines K562 and K-Gla that did not respond to TPA with terminal macrophage differentiation. The treatment of HL-60 and U-937 cell lines with 1-oleoyl-2-acetylglycerol, a synthetic analog of diacylglycerol that, like TPA, stimulates protein kinase C activity, was followed by early and transient induction of c-fos mRNA in the absence of terminal macrophage differentiation. Finally, treatment of HL-60 with TPA in the presence of retinal, an inhibitor of protein kinase C, drastically reduced the induction of c-fos mRNA but had no effect on the terminal macrophage differentiation that is induced in this cell line by TPA. These results indicate that the induction of c-fos and terminal macrophage differentiation in response to TPA treatment can be dissociated in the in vitro models provided by human myeloid leukemic cell lines. Moreover, these findings suggest that the induction of c-fos is not only insufficient but may also be unnecessary for the differentiation along the monocyte-macrophage pathway.
用佛波酯（TPA）处理五种人类髓系白血病细胞系（KG1、ML3、HL - 60、U - 937和HEL）后，细胞发生巨噬细胞分化，并伴随着原癌基因c - fos的mRNA水平早期短暂升高。在对TPA无终末巨噬细胞分化反应的人类细胞系K562和K - Gla中也观察到了c - fos的诱导。用1 - 油酰 - 2 - 乙酰甘油（一种二酰基甘油的合成类似物，与TPA一样刺激蛋白激酶C活性）处理HL - 60和U - 937细胞系后，在无终末巨噬细胞分化的情况下，c - fos mRNA早期短暂诱导。最后，在蛋白激酶C抑制剂视黄醛存在下用TPA处理HL - 60，显著降低了c - fos mRNA的诱导，但对TPA在此细胞系中诱导的终末巨噬细胞分化没有影响。这些结果表明，在人类髓系白血病细胞系提供的体外模型中，对TPA处理的c - fos诱导和终末巨噬细胞分化可以分离。此外，这些发现表明，c - fos的诱导不仅不足以促进单核细胞 - 巨噬细胞途径的分化，而且可能也是不必要的。
