Bienz M, Pelham H R
Cell. 1986 Jun 6;45(5):753-60. doi: 10.1016/0092-8674(86)90789-0.
The Xenopus hsp70 promoter contains three copies of the consensus heat shock element (HSE) between positions -260 and -100. When the gene is transfected into mammalian cells, maximal heat-induced expression requires two HSEs in addition to a CCAAT box located next to the TATA box. The HSE-containing region can be separated from the CCAAT/TATA region without affecting expression of the gene, and it can enhance transcription of a linked beta-globin gene upon heat shock. It thus has the properties of a heat-inducible enhancer. Such an enhancer can also be generated by duplication of HSE sequences from the Drosophila hsp70 promoter, which were previously identified as an upstream promoter element and are known to bind a purified heat shock transcription factor in vitro.
非洲爪蟾hsp70启动子在-260至-100位之间含有三个共有热休克元件（HSE）拷贝。当该基因转染到哺乳动物细胞中时，最大热诱导表达除了需要位于TATA框旁边的一个CCAAT框外，还需要两个HSE。含HSE的区域可以与CCAAT/TATA区域分离而不影响基因表达，并且在热休克时它可以增强相连的β-珠蛋白基因的转录。因此它具有热诱导增强子的特性。这样的增强子也可以通过重复果蝇hsp70启动子的HSE序列产生，这些序列先前被鉴定为上游启动子元件，并且已知在体外与纯化的热休克转录因子结合。
