Reijne Aaffien C, Talarovicova A, Ciapaite Jolita, Bruggink J E, Bleeker A, Groen Albert K, Reijngoud Dirk-Jan, Bakker Barbara M, van Dijk Gertjan
Groningen Institute for Evolutionary Life Sciences, Dept of Behavioral Neuroscience, University of Groningen, Groningen, The Netherlands.
Department of Pediatrics, Center for Liver, Digestive and Metabolic Diseases, University of Groningen, University Medical Center Groningen, Groningen, The Netherlands.
Aging (Albany NY). 2019 Mar 11;11(5):1564-1579. doi: 10.18632/aging.101857.
Diet and physical activity are thought to affect sustainable metabolic health and survival. To improve understanding, we studied survival of mice feeding a low-fat (LF) or high-saturated fat/high sugar (HFS) diet, each with or without free running wheel (RW) access. Additionally several endocrine and metabolic health indices were assessed at 6, 12, 18 and 24 months of age. As expected, HFS feeding left-shifted survival curve of mice compared to LF feeding, and this was associated with increased energy intake and increased (visceral/total) adiposity, liver triglycerides, and increased plasma cholesterol, corticosterone, HOMA-IR, and lowered adiponectin levels. Several of these health parameters improved (transiently) by RW access in HFS and LF fed mice (i.e., HOMA-IR, plasma corticosterone), others however deteriorated (transiently) by RW access only in HFS-fed mice (i.e., body adiposity, plasma resistin, and free cholesterol levels). Apart from these multiple and sometimes diverging health effects of RW access, RW access did not affect survival curves. Important to note, voluntary RW activity declined with age, but this effect was most pronounced in the HFS fed mice. These results thus challenge the hypothesis that voluntary wheel running can counteract HFS-induced deterioration of survival and metabolic health.
饮食和身体活动被认为会影响可持续的代谢健康和生存期。为了增进了解，我们研究了喂食低脂（LF）或高饱和脂肪/高糖（HFS）饮食的小鼠的生存期，每种饮食条件下小鼠均可自由使用跑步轮（RW）或不使用。此外，在6、12、18和24月龄时评估了多项内分泌和代谢健康指标。正如预期的那样，与喂食LF饮食相比，喂食HFS饮食使小鼠的生存曲线左移，这与能量摄入增加、（内脏/总体）肥胖增加、肝脏甘油三酯增加、血浆胆固醇、皮质酮、HOMA-IR增加以及脂联素水平降低有关。在喂食HFS和LF饮食的小鼠中，通过使用RW，这些健康参数中的一些得到了（短暂）改善（即HOMA-IR、血浆皮质酮），然而，仅在喂食HFS饮食的小鼠中，通过使用RW，其他一些参数（短暂）恶化（即身体肥胖、血浆抵抗素和游离胆固醇水平）。除了使用RW带来的这些多重且有时相互矛盾的健康影响外，使用RW并未影响生存曲线。需要注意的是，自愿的RW活动随年龄下降，但这种影响在喂食HFS饮食的小鼠中最为明显。因此，这些结果对自愿跑步可以抵消HFS诱导的生存期和代谢健康恶化这一假设提出了挑战。
