Centre for Protolife Research and Centre for Organized Matter Chemistry, School of Chemistry, University of Bristol, Bristol, BS8 1TS, UK.
Angew Chem Int Ed Engl. 2019 May 6;58(19):6333-6337. doi: 10.1002/anie.201901469. Epub 2019 Apr 2.
Collective behaviour in mixed populations of synthetic protocells is an unexplored area of bottom-up synthetic biology. The dynamics of a model protocell community is exploited to modulate the function and higher-order behaviour of mixed populations of bioinorganic protocells in response to a process of artificial phagocytosis. Enzyme-loaded silica colloidosomes are spontaneously engulfed by magnetic Pickering emulsion (MPE) droplets containing complementary enzyme substrates to initiate a range of processes within the host/guest protocells. Specifically, catalase, lipase, or alkaline phosphatase-filled colloidosomes are used to trigger phagocytosis-induced buoyancy, membrane reconstruction, or hydrogelation, respectively, within the MPE droplets. The results highlight the potential for exploiting surface-contact interactions between different membrane-bounded droplets to transfer and co-locate discrete chemical packages (artificial organelles) in communities of synthetic protocells.
混合群体的合成原细胞的集体行为是一个未被探索的自下而上的合成生物学领域。利用模型原细胞群落的动力学来调节混合群体的生物无机原细胞的功能和更高阶行为，以响应人工吞噬作用的过程。酶负载的二氧化硅胶体囊泡会自发地被含有互补酶底物的磁性 Pickering 乳液 (MPE) 液滴吞噬，从而在主体/客体原细胞内引发一系列过程。具体来说，用过氧化氢酶、脂肪酶或碱性磷酸酶填充的胶体囊泡分别用于触发吞噬作用诱导的浮力、膜重建或水凝胶化，这是在 MPE 液滴内发生的。研究结果强调了利用不同膜结合液滴之间的表面接触相互作用来转移和共定位离散化学包（人工细胞器）在合成原细胞群落中的潜力。
