老年受试者静息和活化的无血小板单核细胞中前列腺素和血栓烷的生物合成

Prostaglandin and thromboxane biosynthesis in resting and activated platelet-free monocytes from aged subjects.

作者信息

Chiricolo M, Bartolini G, Orlandi M, Peta G, Corneli M, Spinosa M, Tomasi V, Franceschi C

出版信息

Gerontology. 1986;32(2):69-73. doi: 10.1159/000212768.

Abstract

The main cyclo-oxygenase-dependent arachidonic acid (AA) derivatives, i.e. prostaglandin E2 (PGE2) and thromboxane A2 (TXA2), have been measured by radioimmunoassay in platelet-free cultures of human monocytes from young and old subjects, in presence and in absence of activating substances (10% fetal calf serum). No difference was found between cells from the two groups as far as the production of PGE2 and TXB2 (stable metabolite of TXA2) was concerned, at variance with reported data in young and old experimental animals. The addition to the cultures of exogenous AA caused a reorientation of cyclic endoperoxide metabolism resulting in a consistent decrease of the ratio TXB2/PGE2, but only in monocytes from young subjects. The data are discussed with respect to the claimed role of prostaglandins in the age-related immune derangement which is present in aged humans.

摘要

已通过放射免疫分析法，在有和没有激活物质（10%胎牛血清）存在的情况下，对年轻和老年受试者的人单核细胞无血小板培养物中主要的环氧化酶依赖性花生四烯酸（AA）衍生物，即前列腺素E2（PGE2）和血栓素A2（TXA2）进行了测定。就PGE2和TXB2（TXA2的稳定代谢产物）的产生而言，两组细胞之间未发现差异，这与年轻和老年实验动物的报道数据不同。向培养物中添加外源性AA会导致环内过氧化物代谢重新定向，从而使TXB2/PGE2比值持续降低，但仅在年轻受试者的单核细胞中出现这种情况。针对前列腺素在老年人类中存在的与年龄相关的免疫紊乱中所声称的作用，对这些数据进行了讨论。