Nichols F C, Schenkein H A, Rutherford R B
Biochim Biophys Acta. 1987 Feb 18;927(2):149-57. doi: 10.1016/0167-4889(87)90128-5.
C3b or lipopolysaccharide treatment of human peripheral blood monocytes in culture stimulates an early release of thromboxane B2 and a delayed release of prostaglandin E into culture supernatants. Immunoreactive thromboxane B2 release is maximal from 2-8 h, whereas prostaglandin E release is maximal from 16-24 h after stimulation of monocytes in culture. We further examined this process by comparing the time course of labelled prostaglandin E2, prostaglandin E1 and thromboxane B2 release from human monocytes which were pulse or continuously labelled with [3H]arachidonic acid and [14C]eicosatrienoic acid. The release of labelled eicosanoids was compared with the release of immunoreactive prostaglandin E and thromboxane B2. The time course of prostaglandin E2 release was virtually identical to the release of prostaglandin E1 in all culture supernatants regardless of labelling conditions. However, release of immunoreactive prostaglandin E paralleled the release of labelled prostaglandin E1 and E2 only for continuously labelled cultures. The release of labelled prostaglandin E1 and E2 from pulse labelled cultures paralleled the release of thromboxane B2 and not immunoreactive prostaglandin. In contrast, labelled and immunoreactive thromboxane B2, quantitated in the same culture supernatants, demonstrated similar release patterns regardless of labelling conditions. These findings indicate that the differential pattern of prostaglandin E and thromboxane B2 release from human monocytes is not related to a time-dependent shift in the release of prostaglandin E1 relative to prostaglandin E2. Because thromboxane B2 and prostaglandin E2 are produced through cyclooxygenase mediated conversion of arachidonic acid, these results further suggest that prostaglandin E2 and thromboxane B2 are independently metabolized in human monocyte populations.
在培养过程中，用C3b或脂多糖处理人外周血单核细胞，可刺激血栓素B2早期释放，并使前列腺素E延迟释放到培养上清液中。免疫反应性血栓素B2的释放在刺激单核细胞培养后2 - 8小时达到最大值，而前列腺素E的释放在16 - 24小时达到最大值。我们通过比较用[3H]花生四烯酸和[14C]二十碳三烯酸脉冲标记或连续标记的人单核细胞中标记的前列腺素E2、前列腺素E1和血栓素B2释放的时间进程，进一步研究了这一过程。将标记类花生酸的释放与免疫反应性前列腺素E和血栓素B2的释放进行比较。无论标记条件如何，在所有培养上清液中，前列腺素E2的释放时间进程与前列腺素E1的释放几乎相同。然而，免疫反应性前列腺素E的释放仅在连续标记的培养物中与标记的前列腺素E1和E2的释放平行。脉冲标记培养物中标记的前列腺素E1和E2的释放与血栓素B2的释放平行，而与免疫反应性前列腺素不平行。相反，在相同培养上清液中定量的标记和免疫反应性血栓素B2，无论标记条件如何，都显示出相似的释放模式。这些发现表明，人单核细胞中前列腺素E和血栓素B2释放的差异模式与前列腺素E1相对于前列腺素E2释放的时间依赖性变化无关。由于血栓素B2和前列腺素E2是通过环氧化酶介导的花生四烯酸转化产生的，这些结果进一步表明前列腺素E2和血栓素B2在人单核细胞群体中是独立代谢的。
