Yates I E, Bacon C W, Hinton D M
Toxicology and Mycotoxin Research Unit, Richard B. Russell Agricultural Research Center, USDA/ARS, P.O. Box 5677, Athens, GA 30604.
Plant Dis. 1997 Jul;81(7):723-728. doi: 10.1094/PDIS.1997.81.7.723.
Kernels of corn, Zea mays, were inoculated with Fusarium moniliforme to analyze seedling growth and development during endophytic, symptomless infection. In planta F. moniliforme distribution and seedling growth, expressed as shoot diameter, plant height, leaf length, and dry weight, were examined weekly for 28 days after planting. Even though no visible disease symptoms developed, F. moniliforme was isolated from most segments taken from seedlings grown from inoculated, but not noninoculated, kernels from the earliest to the latest sampling. F. moniliforme did not alter the rate or percentage of kernel germination, but seedlings grown from inoculated kernels had suppressed shoot diameter, plant height, leaf length, and plant weight 7 days after planting. However, seedling growth from inoculated kernels was similar to or greater than that from noninoculated kernels at 28 days. Histological modifications in seedlings grown from inoculated kernels included accelerated lignin deposition in shoots and modified chloroplast orientation in leaves. In summary, gross morphology and histology were altered in corn seedlings during symptomless, endophytic infection by F. moniliforme.
将玉米（Zea mays）的籽粒接种串珠镰刀菌（Fusarium moniliforme），以分析内生无症状感染期间幼苗的生长发育情况。种植后28天内，每周检查一次植株内串珠镰刀菌的分布情况以及幼苗的生长情况，幼苗生长情况以茎直径、株高、叶长和干重表示。尽管未出现明显的病害症状，但从接种籽粒（而非未接种籽粒）长成的幼苗上采集的大部分切段，从最早到最晚采样时间，均分离出了串珠镰刀菌。串珠镰刀菌并未改变籽粒的发芽率或发芽百分比，但接种籽粒长成的幼苗在种植7天后，其茎直径、株高、叶长和植株重量均受到抑制。然而，在28天时，接种籽粒长成的幼苗生长情况与未接种籽粒长成的幼苗相似或更好。接种籽粒长成的幼苗的组织学变化包括茎中木质素沉积加速以及叶片中叶绿体方向改变。总之，在串珠镰刀菌无症状内生感染期间，玉米幼苗的总体形态和组织学发生了改变。
