Leone Federica, Cataldo Roberta, Mohamed Sara S Y, Manna Luigi, Banchero Mauro, Ronchetti Silvia, Mandras Narcisa, Tullio Vivian, Cavalli Roberta, Onida Barbara
Politecnico di Torino, Department of Applied Science and Technology, Corso Duca Degli Abruzzi 24, 10129 Turin, Italy.
Department of Public Health and Pediatrics, Microbiology Division, University of Turin, via Santena 9, 10126 Turin, Italy.
Nanomaterials (Basel). 2019 Mar 11;9(3):407. doi: 10.3390/nano9030407.
The physico⁻chemical and biological properties of nanostructured ZnO are combined with the non-toxic and eco-friendly features of the scCO₂-mediated drug loading technique to develop a multifunctional antimicrobial drug delivery system for potential applications in wound healing. Two nanostructured ZnO (NsZnO) with different morphologies were prepared through wet organic-solvent-free processes and characterized by means of powder X-ray diffraction, field emission scanning electron microscopy (FESEM), and nitrogen adsorption analysis. The antimicrobial activity of the two samples against different microbial strains was investigated together with the in vitro Zn release. The results indicated that the two ZnO nanostructures exhibited the following activity: > . A correlation between the antimicrobial activity, the physico⁻chemical properties (specific surface area and crystal size) and the Zn ion release was found. Ibuprofen was, for the first time, loaded on the NsZnO carriers with a supercritical CO₂-mediated drug impregnation process and in vitro dissolution studies of the loaded drug were performed. A successful loading up to 14% / of ibuprofen in its amorphous form was obtained. A preliminary drug release test showed that up to 68% of the loaded ibuprofen could be delivered to a biological medium, confirming the feasibility of using NsZnO as a multifunctional antimicrobial drug carrier.
纳米结构氧化锌的物理化学和生物学特性与超临界二氧化碳介导的药物负载技术的无毒及环保特性相结合，以开发一种多功能抗菌药物递送系统，用于伤口愈合的潜在应用。通过无有机溶剂湿法制备了两种具有不同形貌的纳米结构氧化锌（NsZnO），并通过粉末X射线衍射、场发射扫描电子显微镜（FESEM）和氮吸附分析对其进行了表征。研究了这两种样品对不同微生物菌株的抗菌活性以及体外锌释放情况。结果表明，这两种氧化锌纳米结构表现出以下活性：> 。发现了抗菌活性、物理化学性质（比表面积和晶体尺寸）与锌离子释放之间的相关性。首次采用超临界二氧化碳介导的药物浸渍工艺将布洛芬负载到NsZnO载体上，并对负载药物进行了体外溶出研究。成功获得了高达14%的无定形布洛芬负载量。初步药物释放试验表明，高达68%的负载布洛芬可递送至生物介质，证实了使用NsZnO作为多功能抗菌药物载体的可行性。
