Thatcher Louise F, Singh Karam B
CSIRO Agriculture and Food, Centre for Environment and Life Sciences, Wembley, Western Australia 6913, Australia.
The Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, Western Australia 6009, Australia.
Plants (Basel). 2019 Mar 11;8(3):60. doi: 10.3390/plants8030060.
The () gene is recognised as a marker for early defence and stress responses. To identify regulators of these responses, a forward genetic screen for Arabidopsis mutants with up-regulated promoter activity was conducted by screening a mutagenized population containing a promoter fragment fused to the luciferase reporter gene (). We previously identified several () mutants from this screen that conferred constitutive activity and increased resistance to several pathogens and/or insects pests. Here we identified a further mutant constitutively expressing and termed (). Unlike the mutants, was more susceptible to disease symptom development induced by two necrotrophic fungal pathogens; the root pathogen and the leaf pathogen . The allele was mapped to a 2.1 Mbp region of chromosome 2 and narrowed to four candidate loci.
（）基因被认为是早期防御和应激反应的标志物。为了鉴定这些反应的调节因子，通过筛选含有与荧光素酶报告基因（）融合的启动子片段的诱变群体，对拟南芥突变体进行了正向遗传筛选，这些突变体具有上调的启动子活性。我们之前从该筛选中鉴定出了几个（）突变体，它们具有组成型活性，并增强了对几种病原体和/或害虫的抗性。在这里，我们鉴定出了另一个组成型表达（）的突变体，并将其命名为（）。与（）突变体不同，（）对两种坏死营养型真菌病原体诱导的疾病症状发展更敏感；根部病原体（）和叶片病原体（）。（）等位基因被定位到2号染色体的一个2.1 Mbp区域，并缩小到四个候选基因座。
