Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, College of Life Sciences, Northwest University, Xi'an, 710069, Shaanxi, China.
Planta. 2019 Jun;249(6):1889-1902. doi: 10.1007/s00425-019-03130-5. Epub 2019 Mar 12.
MYB15 promoter of Vitis quinquangularis has potential as a target for disease resistance breeding, and its involvement in PTI is associated with a range of defense mechanisms. China is a center of origin for Vitis and is home to diverse wild Vitis genotypes, some of which show superior pathogen resistance, although the underlying molecular basis for this has not yet been elucidated. In the current study, we identified a transcription factor, MYB15, from the Chinese wild grape, Vitis quinquangularis, whose promoter region (pVqMYB15) was shown to be induced by basal immunity (also called PAMP-triggered immunity, PTI) triggered by flg22, following heterologous expression in Nicotiana benthamiana and homologous expression in grapevine. By analyzing the promoter structure and activity, we identified a unique 283 bp sequence that plays a key role in the activation of basal immunity. In addition, we showed that activation of the MYB15 promoter correlates with differences in the expression of MYB15 and RESVERATROL SYNTHASE (RS) induced by the flg22 elicitor. We further tested whether the MYB15 induction triggered by flg22 was consistent with MYB15 and RS expression following inoculation with Plasmopara viticola in grape (V. quinquangularis and Vitis vinifera) leaves. Mapping upstream signals, we found that calcium influx, an RboH-dependent oxidative burst, an MAPK cascade, and jasmonate and salicylic acid co-contributed to flg22-triggered pVqMYB15 activation. Our data suggest that the MYB15 promoter has potential as a target for disease resistance breeding, and its involvement in PTI is associated with a range of defense mechanisms.
五倍子葡萄 MYB15 启动子有望成为抗病育种的靶标，其参与 PTI 与一系列防御机制有关。中国是葡萄的起源中心，拥有丰富的野生葡萄基因型，其中一些表现出对病原体的抗性优势，尽管其潜在的分子基础尚未阐明。在本研究中，我们从中国野生葡萄五倍子中鉴定出一个转录因子 MYB15，其启动子区域（pVqMYB15）在 flagellin22 触发的基础免疫（也称为 PAMP 触发免疫，PTI）后被诱导，在 Nicotiana benthamiana 中异源表达和葡萄中同源表达。通过分析启动子结构和活性，我们确定了一个独特的 283bp 序列，该序列在基础免疫的激活中起关键作用。此外，我们表明，MYB15 启动子的激活与 flagellin22 诱导的 MYB15 和 RESVERATROL SYNTHASE（RS）表达的差异相关。我们进一步测试了 flagellin22 诱导的 MYB15 诱导是否与葡萄（五倍子和 Vitis vinifera）叶片接种 Plasmopara viticola 后 MYB15 和 RS 表达一致。我们发现钙流入、RboH 依赖性氧化爆发、MAPK 级联、茉莉酸和水杨酸共同作用于 flagellin22 触发的 pVqMYB15 激活，从而映射上游信号。我们的数据表明，MYB15 启动子有望成为抗病育种的靶标，其参与 PTI 与一系列防御机制有关。
