Brott Ashley S, Sychantha David, Clarke Anthony J
Department of Molecular and Cellular Biology, University of Guelph, Guelph, ON, Canada.
Methods Mol Biol. 2019;1954:115-136. doi: 10.1007/978-1-4939-9154-9_10.
The polysaccharides that comprise bacterial cell walls are commonly O-acetylated. This modification confers resistance to hydrolases of innate immune systems and/or controls endogenous autolytic activity. Herein, we present protocols for the compositional analysis of bacterial cell wall O-acetylation, and assays for monitoring O-acetyltransferases and O-acetylesterases. The assays are amenable for the development of high-throughput screens in search of inhibitors of the respective enzymes.
构成细菌细胞壁的多糖通常会发生O-乙酰化修饰。这种修饰赋予细菌对先天免疫系统水解酶的抗性和/或控制内源性自溶活性。在此，我们提供了用于细菌细胞壁O-乙酰化成分分析的方案，以及监测O-乙酰转移酶和O-乙酰酯酶的检测方法。这些检测方法适用于开发高通量筛选，以寻找相应酶的抑制剂。
