Abukar Tasnim, Buenbrazo Nakita, Janesch Bettina, Kell Laura, Wakarchuk Warren
Department of Chemistry and Biology, Ryerson University, Toronto, ON, Canada.
Methods Mol Biol. 2019;1954:215-235. doi: 10.1007/978-1-4939-9154-9_17.
Glycans play many important roles in bacterial biology and the complexity of the glycan structures requires biochemical assays in place to help characterize the biosynthetic pathways. Our focus has been on the use of enzymes from pathogens which make molecular mimics of host glycans. We have been examining glycosyltransferases that make strategic linkages in biologically active glycans which can be also exploited for potential therapeutic glycoconjugate synthesis. This chapter will provide details on assays for a variety of bacterial glycosyltransferases that we and others have used for the characterization of pathogen glycoconjugate biosynthetic pathways, and for the in vitro synthesis of human-like glycans produced by bacterial pathogens. The methods presented here should enable other assays to be developed for new pathway characterization.
聚糖在细菌生物学中发挥着许多重要作用，聚糖结构的复杂性需要合适的生化分析方法来帮助表征生物合成途径。我们一直专注于利用病原体中的酶，这些酶可制造宿主聚糖的分子模拟物。我们一直在研究糖基转移酶，它们在生物活性聚糖中形成关键连接，这些连接也可用于潜在治疗性糖缀合物的合成。本章将详细介绍我们及其他人用于表征病原体糖缀合物生物合成途径以及体外合成细菌病原体产生的类人聚糖的各种细菌糖基转移酶的分析方法。这里介绍的方法应能促使开发用于新途径表征的其他分析方法。
