体外评估益生元活性。
In Vitro Assessment of Prebiotic Activity.
机构信息
Department of Life Sciences, University of Modena and Reggio Emilia, Modena, Italy.
BIOGEST-SITEIA, University of Modena and Reggio Emilia, Modena, Italy.
出版信息
Methods Mol Biol. 2021;2278:209-223. doi: 10.1007/978-1-0716-1274-3_17.
Bifidogenic effect is a main target for the assessment of prebiotic activity. pH-controlled batch processes of bifidobacteria and fecal microbiota are herein presented. Growth of bifidobacteria, carbohydrate breakdown and consumption, organic acid production, and activity of specific glycosyl hydrolases involved in the hydrolysis of di-, oligo-, or polysaccharides are exploited to study and compare substrate preference of bifidobacteria for candidate prebiotics.
双歧因子效应是评估益生元活性的主要目标。本文介绍了双歧杆菌和粪便微生物群的 pH 控制间歇培养。利用双歧杆菌的生长、碳水化合物的分解和消耗、有机酸的产生以及参与二糖、寡糖或多糖水解的特定糖苷水解酶的活性，研究和比较了双歧杆菌对候选益生元的底物偏好。
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