Department of Virology, Kimron Veterinary Institute, Bet Dagan, Israel.
"Hachaklait", Mutual Society for Veterinary Services, Caesaria, Israel.
Transbound Emerg Dis. 2019 May;66(3):1126-1131. doi: 10.1111/tbed.13167. Epub 2019 Mar 29.
The insect-transmitted Shuni virus (SHUV) belongs to the Simbu serogroup of orthobunyaviruses and it is known to induce abortions, stillbirths and severe congenital malformations in ruminants and may cause neurological signs in infected horses. Here, SHUV was detected in brain samples of two Israeli cattle, which suffered from severe neurological signs that led to the deaths of the animals. During histopathological examination of the first case, a 5-month-old calf, small perivascular cuffs, composed mainly of neutrophils with few lymphocytes were observed in the brain stem and cerebrum. Similar infiltrates were also found to a lesser extent in the cerebellar meninges leading to the diagnosis of acute-subacute meningoencephalitis. The histological examination of the brainstem from the second case, a 16-month-old heifer, revealed perivascular infiltration composed of equal numbers of macrophages and neutrophils associated with cerebral and meningeal haemorrhages. In this case encephalitis was diagnosed. Viral RNA was extracted from brain samples of both cattle that suffered from severe neurological signs and was subsequently tested by a polymerase chain reaction PCR assay specific for Simbu serogroup viruses and found positive. The presence of SHUV was subsequently confirmed by the isolation of the virus from one sample and sequence analysis of both brain samples. The comparison of the complete sequences of the coding regions of all three genome segments from both cases revealed a close relationship to Shuni viruses detected in tissue samples of aborted or malformed calves or lambs born during the last years in Israel.
昆虫传播的舒尼病毒（SHUV）属于辛布血清群正粘病毒，已知会导致反刍动物流产、死产和严重先天畸形，并可能导致感染马出现神经症状。在这里，在两只以色列牛的脑组织样本中检测到了 SHUV，这两只牛患有严重的神经症状，导致动物死亡。在首例病例（一头 5 月龄小牛）的组织病理学检查中，观察到脑干和大脑中有主要由中性粒细胞和少量淋巴细胞组成的小血管周围套袖。在小脑脑膜中也发现了类似的浸润，但程度较轻，导致急性-亚急性脑膜脑炎的诊断。第二例病例（一头 16 月龄小母牛）的脑干组织学检查显示，血管周围浸润由数量相等的巨噬细胞和中性粒细胞组成，伴有脑和脑膜出血。在这种情况下，诊断为脑炎。从两只患有严重神经症状的牛的脑组织样本中提取病毒 RNA，随后通过针对辛布血清群病毒的聚合酶链反应（PCR）检测呈阳性。随后从一份样本中分离出病毒，并对两份脑组织样本进行序列分析，确认了 SHUV 的存在。对两例病例所有三个基因组片段编码区的完整序列进行比较，发现与过去几年在以色列出生的流产或畸形小牛或羔羊组织样本中检测到的舒尼病毒密切相关。
