Golender Natalia, Wernike Kerstin, Bumbarov Velizar, Aebischer Andrea, Panshin Alexander, Jenckel Maria, Khinich Yevgeny, Beer Martin
Divisions of Virology, Kimron Veterinary Institute, 50250, Bet Dagan, Israel.
Institute of Diagnostic Virology, Friedrich-Loeffler-Institut, 17493, Greifswald-Insel Riems, Germany.
Virus Genes. 2016 Dec;52(6):806-813. doi: 10.1007/s11262-016-1381-3. Epub 2016 Aug 19.
Shuni virus (SHUV) was recently identified in Israel in several brains of ovine, bovine, and goat fetuses and newborn animals with congenital arthrogryposis-hydranencephaly syndrome. In the present study, the sequences of several Israeli SHUV strains were analyzed in detail; based on the small genome segment which encodes the nucleocapsid protein and the small nonstructural protein (NSs), a very high similarity of 99-100 % among each other was found. In contrast to the highly conserved N protein, several mutations were found within the NSs-coding sequence of SHUVs present in brain samples of malformed fetuses, resulting in a considerably frequent appearance of stop codons. Interferon alpha/beta production was demonstrated in an in-vitro interferon bioassay; hence, the virus isolated from the brain of a malformed sheep fetus acquired mutations, resulting in the loss of its NSs protein function.
舒尼病毒（SHUV）最近在以色列的几只患有先天性关节弯曲-积水性无脑综合征的绵羊、牛和山羊胎儿及新生动物的大脑中被发现。在本研究中，对几株以色列SHUV毒株的序列进行了详细分析；基于编码核衣壳蛋白和小非结构蛋白（NSs）的小基因组片段，发现它们彼此之间具有99%-100%的高度相似性。与高度保守的N蛋白不同，在畸形胎儿脑样本中存在的SHUV的NSs编码序列内发现了几个突变，导致终止密码子相当频繁地出现。在体外干扰素生物测定中证实了α/β干扰素的产生；因此，从一只畸形绵羊胎儿大脑中分离出的病毒发生了突变，导致其NSs蛋白功能丧失。
