Dörken B, Moldenhauer G, Pezzutto A, Schwartz R, Feller A, Kiesel S, Nadler L M
J Immunol. 1986 Jun 15;136(12):4470-9.
The B cell-restricted antigen HD39, whose cell surface expression is limited to resting and activated human B lymphocytes, is described in this report. The monoclonal antibody HD39 detects a two-chain glycoprotein with apparent molecular weights of 130,000 and 140,000. During B cell ontogeny, HD39 is first expressed in the cytoplasm of bone marrow derived pre-B cells, then appears on the cell surface of sIgM+ B cells, and finally on the majority of sIgM+ sIgD+ resting B cells. After activation in vitro, the expression of HD39 on the cell surface first increases, and then the antigen is lost as cells begin to differentiate. HD39 is weakly expressed on very few non-T cell ALL and B cell CLL, on approximately 50% of B cell lymphomas, and not on Waldenström's macroglobulinemias and myelomas. In contrast, it is strongly expressed on all hairy cell leukemias. Its limited cell surface expression in B cell ontogeny suggests that HD39 may be important in the events that regulate the activation of the human resting B lymphocytes.
本报告描述了B细胞限制性抗原HD39，其细胞表面表达仅限于静止和活化的人B淋巴细胞。单克隆抗体HD39可检测到一种双链糖蛋白，其表观分子量为130,000和140,000。在B细胞个体发育过程中，HD39首先在骨髓来源的前B细胞的细胞质中表达，然后出现在sIgM+B细胞的细胞表面，最后出现在大多数sIgM+sIgD+静止B细胞上。体外激活后，HD39在细胞表面的表达首先增加，然后随着细胞开始分化而丢失该抗原。HD39在极少数非T细胞急性淋巴细胞白血病和B细胞慢性淋巴细胞白血病中弱表达，在约50%的B细胞淋巴瘤中表达，而在华氏巨球蛋白血症和骨髓瘤中不表达。相反，它在所有毛细胞白血病中强烈表达。其在B细胞个体发育中有限的细胞表面表达表明，HD39可能在调节人静止B淋巴细胞活化的事件中起重要作用。
