Laboratory of Plant and Process Design, Department of Biochemical and Chemical Engineering , TU Dortmund University , Emil-Figge-Straße 70 , D-44227 Dortmund , Germany.
Phytowelt Green Technologies GmbH , Kölsumer Weg 33 , D-41334 Nettetal , Germany.
J Agric Food Chem. 2019 Dec 11;67(49):13412-13419. doi: 10.1021/acs.jafc.8b07270. Epub 2019 Mar 22.
Recently, the market value of aromas has constantly been rising. Because the supply from natural feedstock is limited, the biotechnological production has received more interest. Thus far, only a few attempts have been made to produce α-ionone, a valued essential aroma of raspberry, biotechnologically. This study reports a production process for enantiopure ()-α-ionone from lab scale (2-150 L) with typical titer of 285 mg/L broth to industrial scale (up to 10 000 L) with a titer up to 400 mg/L broth, focusing on the development of a downstream process with a maximized yield at minimized effort. The developed recovery consists of solid-liquid extraction from the biomass at φ = 0.4 g of -hexane/g of biomass for 90 min at ambient temperature and adsorption from the aqueous supernatant at Φ = 0.5 g of Diaion HP-20/mg of α-ionone, followed by desorption at Ψ = 30 g of -hexane/g of Diaion HP-20. Altogether, natural α-ionone could be gained in substantial quantity and purity of >95%.
最近，香气的市场价值一直在不断上升。由于天然原料的供应有限，生物技术生产受到了更多的关注。到目前为止，只有少数尝试用生物技术生产覆盆子有价值的香气成分α-紫罗兰酮。本研究报告了一种从实验室规模（2-150 L）到工业规模（高达 10000 L）生产手性纯（）-α-紫罗兰酮的生产工艺，典型的发酵液滴度为 285 mg/L，发酵液滴度高达 400 mg/L，重点是开发一种在最小化努力的情况下最大限度地提高收率的下游工艺。开发的回收工艺包括在室温下用 0.4 g -己烷/g生物质进行固液萃取 90 分钟，以及用 0.5 g Diaion HP-20/mg α-紫罗兰酮从水相上清液中吸附，然后用 30 g -己烷/g Diaion HP-20 解吸。总的来说，可以以>95%的高纯度和大量获得天然α-紫罗兰酮。
