Rezende Gabriela, Arthur Rodrigo Alex, Lamers Marcelo Lazzaron, Hashizume Lina Naomi
Department of Preventive and Social Dentistry, Faculty of Dentistry, UFRGS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Department of Morphological Sciences, Institute of Basic Health Sciences, UFRGS - Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
Braz Dent J. 2019 Jan-Feb;30(1):36-42. doi: 10.1590/0103-6440201902183.
Maltodextrins, derived from corn starch, have been added to industrialized food combined with sucrose. However it is not clear the diffusion properties of the dental biofilm matrix and the tridimensional structure of multispecies biofilms formed in the presence of these carbohydrates. Therefore, the aim of study was to investigate by confocal laser scanning microscopy (CLSM) the structural organization of the multispecies dental biofilm formed in situ under exposure to sucrose associated to maltodextrin. Adult volunteers wore an intraoral palatal appliance containing bovine enamel blocks. They were instructed to remove the appliance 8 times per day and drop the following solutions on the enamel blocks: deionized distilled water (DDW), maltodextrin, sucrose + maltodextrin or sucrose. Biofilms formed were stained and the percentage of extracellular polysaccharide (%EPS) and thickness were determined by CLSM. Biofilm formed in the presence of sucrose and sucrose + maltodextrin presented similar %EPS and higher than DDW and maltodextrin. Regarding to the biofilm thickness, sucrose and sucrose + maltodextrin treatments were thicker than DDW and maltodextrin and similar between them. The structural organization of the multispecies dental biofilm formed in situ in the presence of sucrose does not change when this carbohydrate is associated to maltodextrin.
源自玉米淀粉的麦芽糊精已被添加到与蔗糖混合的工业化食品中。然而，尚不清楚牙菌斑生物膜基质的扩散特性以及在这些碳水化合物存在下形成的多物种生物膜的三维结构。因此，本研究的目的是通过共聚焦激光扫描显微镜（CLSM）研究在与麦芽糊精相关的蔗糖暴露下原位形成的多物种牙菌斑生物膜的结构组织。成年志愿者佩戴含有牛牙釉质块的口腔上颚矫治器。他们被指示每天取出矫治器8次，并将以下溶液滴在牙釉质块上：去离子蒸馏水（DDW）、麦芽糊精、蔗糖+麦芽糊精或蔗糖。对形成的生物膜进行染色，并通过CLSM测定细胞外多糖百分比（%EPS）和厚度。在蔗糖和蔗糖+麦芽糊精存在下形成的生物膜呈现出相似的%EPS，且高于DDW和麦芽糊精。关于生物膜厚度，蔗糖和蔗糖+麦芽糊精处理组比DDW和麦芽糊精处理组更厚，且两者之间相似。当这种碳水化合物与麦芽糊精结合时，在蔗糖存在下原位形成的多物种牙菌斑生物膜的结构组织不会改变。
