Figueira Leandro Wagner, Bessa Muniz Ana, Doria Anelise Cristina Osorio Cesar, Castaldelli Nishime Thalita Mayumi, Kostov Konstantin Georgiev, Koga-Ito Cristiane Y
Department of Environmental Engineering and Oral Biopathology Graduate Program, Institute of Science and Technology, São Paulo State University, UNESP, São José dos Campos, São Paulo, Brazil.
Biotechnology and Electric Plasma Laboratory (Biotechplasma) - Research and Development Institute - IPD - Universidade Do Vale Do Paraíba. Av. Shishima Hifumi, São José dos Campos, Brazil.
J Oral Microbiol. 2024 Sep 9;16(1):2397831. doi: 10.1080/20002297.2024.2397831. eCollection 2024.
This study aimed to determine the effects of low-temperature plasma jet produced in gas helium (LTP-helium) on cariogenic biofilms composedby and , and also by the combination of and . Biofilms were treated for 1, 3, 5, and 7 minutes. A 0.12% chlorhexidine solution was used as the positive control and sterile physiologic solution was the negative control. Biofilm viability was analyzed by viable cell recovery, scanning electron microscopy, and confocal laser scanning microscopy. All assays were performed intriplicate in three independent experiments. Multispecies biofilms exposed to LTP-helium had a significant reduction in viability when compared to the negative control (p < 0.0001). For biofilm formedby , and , LTP treatments for 5 and 7 minutes caused similar reduction of morethan 2 log. Also, a significant reduction in the viability of biofilms formedby , and was detected (p < 0.0001). In conclusion, LTP-helium reduced theviability of cariogenic biofilms with different microbial compositions, which indicates that LTP-helium is a potential tool for developing new protocols for dental caries prevention and treatment.
本研究旨在确定在氦气中产生的低温等离子体射流（LTP-氦气）对由 、 组成以及由 与 组合而成的致龋生物膜的影响。生物膜分别处理1、3、5和7分钟。使用0.12%的氯己定溶液作为阳性对照，无菌生理溶液作为阴性对照。通过活细胞回收率、扫描电子显微镜和共聚焦激光扫描显微镜分析生物膜活力。所有测定在三个独立实验中重复进行三次。与阴性对照相比，暴露于LTP-氦气的多物种生物膜活力显著降低（p < 0.0001）。对于由 、 和 形成的生物膜，5分钟和7分钟的LTP处理导致类似的超过2个对数的降低。此外，还检测到由 、 和 形成的生物膜活力显著降低（p < 0.0001）。总之，LTP-氦气降低了具有不同微生物组成的致龋生物膜的活力，这表明LTP-氦气是开发龋齿预防和治疗新方案的潜在工具。
