Ohishi I, Okada Y
J Gen Microbiol. 1986 Jan;132(1):125-31. doi: 10.1099/00221287-132-1-125.
Botulinum C2 toxin (C2T) is composed of two dissimilar protein components, designated components I and II, which are linked with neither covalent nor noncovalent bonds. The heterogeneity of these two components of C2T produced by Clostridium botulinum type C and D strains was examined. Of 21 strains examined, 19 strains produced the two components, while the others produced neither component I nor component II. The 19 producers of C2T could be divided into three groups based on the differences in antigenicity, molecular weight and biological activity of components I and II. The results provide evidence of heterogeneity in the molecular structure of the two components of C2T, which is possibly a cause of the differences in the biological activity of the toxin observed in different strains.
肉毒杆菌C2毒素（C2T）由两种不同的蛋白质成分组成，分别称为成分I和成分II，它们既没有共价键也没有非共价键相连。对肉毒杆菌C型和D型菌株产生的C2T这两种成分的异质性进行了研究。在所检测的21株菌株中，19株产生了这两种成分，而其他菌株既不产生成分I也不产生成分II。根据成分I和成分II在抗原性、分子量和生物活性方面的差异，这19株C2T产生菌可分为三组。这些结果提供了C2T两种成分分子结构异质性的证据，这可能是在不同菌株中观察到的毒素生物活性差异的一个原因。
