Department of Microbiology and Immunology, University of Minnesota, Minneapolis, MN 55455, USA; Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA.
Center for Immunology, University of Minnesota, Minneapolis, MN 55455, USA; Department of Laboratory Medicine and Pathology, University of Minnesota, Minneapolis, MN 55455, USA.
Cell Rep. 2019 Mar 12;26(11):2859-2867.e4. doi: 10.1016/j.celrep.2019.02.034.
The migratory capacity of adaptive CD8αβ T cells dictates their ability to locate target cells and exert cytotoxicity, which is the basis of immune surveillance for the containment of microbes and disease. The small intestine (SI) is the largest mucosal surface and is a primary site of pathogen entrance. Using two-photon laser scanning microscopy, we found that motility of antigen (Ag)-specific CD8αβ T cells in the SI is dynamic and varies with the environmental milieu. Pathogen-specific CD8αβ T cell movement differed throughout infection, becoming locally confined at memory. Motility was not dependent on CD103 but was influenced by micro-anatomical locations within the SI and by inflammation. CD8 T cells responding to self-protein were initially affected by the presence of self-Ag, but this was altered after complete tolerance induction. These studies identify multiple factors that affect CD8αβ T cell movement in the intestinal mucosa and show the adaptability of CD8αβ T cell motility.
适应性 CD8αβ T 细胞的迁移能力决定了它们定位靶细胞和发挥细胞毒性的能力，这是免疫监视以遏制微生物和疾病的基础。小肠 (SI) 是最大的黏膜表面，也是病原体进入的主要部位。使用双光子激光扫描显微镜，我们发现 SI 中抗原 (Ag)-特异性 CD8αβ T 细胞的运动是动态的，并随环境的变化而变化。病原体特异性 CD8αβ T 细胞的运动在整个感染过程中发生变化，在记忆阶段变得局限于局部。运动不依赖于 CD103，但受 SI 内的微解剖位置和炎症的影响。最初，对自身蛋白作出反应的 CD8 T 细胞受到自身 Ag 的存在的影响，但在完全诱导耐受后，这种情况发生了改变。这些研究确定了影响肠道黏膜中 CD8αβ T 细胞运动的多个因素，并显示了 CD8αβ T 细胞运动的适应性。
