Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical University, No.1 Liyushan Road, Urumqi 830054, Xinjiang, China.
Department of Neurosurgery, The First Affiliated Hospital of Xinjiang Medical University, No.1 Liyushan Road, Urumqi 830054, Xinjiang, China
Biosci Rep. 2019 Apr 2;39(4). doi: 10.1042/BSR20190232. Print 2019 Apr 30.
LINC00599 has been suggested to be involved in physiological and pathological processes including carcinogenesis. However, the clinical and prognostic significance of LINC00599 in glioma patients and the effect of LINC00599 on glioma cell migration and invasion remain unknown. In our results, we first observe the expression of LINC00599 in 31 types of human cancers including tumor tissues and corresponding normal tissues at The Cancer Genome Atlas (TCGA) database, and found that LINC00599 expression levels were only reduced in lower grade glioma (LGG) tissues and glioblastoma multiforme (GBM) tissues compared with normal brain tissues. Moreover, we confirmed levels of LINC00599 expression were decreased in glioma tissues and cell lines compared with matched adjacent normal tissues and normal human astrocytes (NHAs), respectively. Meanwhile, we found that glioma tissues with WHO III-IV grade exhibited lower levels of LINC00599 expression than glioma tissues with I-II grade. The survival analysis at TCGA data showed low LINC00599 expression was associated with poor disease-free survival and overall survival in glioma patients. study suggested up-regulation of LINC00599 depressed glioma cell migration and invasion through regulating epithelial-mesenchymal transition (EMT) process. In conclusion, LINC00599 acts as a tumor-suppressing long non-coding RNA (lncRNA) in glioma.
LINC00599 被认为参与包括癌症发生在内的生理和病理过程。然而，LINC00599 在脑胶质瘤患者中的临床和预后意义以及 LINC00599 对脑胶质瘤细胞迁移和侵袭的影响尚不清楚。在我们的研究结果中，我们首先在癌症基因组图谱（TCGA）数据库中观察到 LINC00599 在 31 种人类癌症中的表达，包括肿瘤组织和相应的正常组织，并发现 LINC00599 的表达水平仅在低级别胶质瘤（LGG）组织和胶质母细胞瘤（GBM）组织中低于正常脑组织。此外，我们分别证实 LINC00599 的表达水平在胶质瘤组织和细胞系中低于匹配的相邻正常组织和正常人星形胶质细胞（NHAs）。同时，我们发现 WHO III-IV 级的胶质瘤组织中 LINC00599 的表达水平低于 I-II 级的胶质瘤组织。TCGA 数据的生存分析表明，LINC00599 低表达与胶质瘤患者无病生存和总生存不良相关。研究表明，LINC00599 的上调通过调节上皮-间充质转化（EMT）过程抑制胶质瘤细胞的迁移和侵袭。总之，LINC00599 在胶质瘤中作为一种肿瘤抑制性长非编码 RNA（lncRNA）发挥作用。
