潜伏型人巨细胞病毒转录组。
The Transcriptome of Latent Human Cytomegalovirus.
机构信息
Department of Molecular Genetics, Weizmann Institute of Science, Rehovot, Israel
出版信息
J Virol. 2019 May 15;93(11). doi: 10.1128/JVI.00047-19. Print 2019 Jun 1.
Abstract
The latent human cytomegalovirus (HCMV) transcriptome has been extremely difficult to define due to the scarcity of naturally latent cells and the complexity of available models. The genomic era offers many approaches to transcriptome profiling that hold great potential for elucidating this challenging issue. The results from two recent studies applying different transcriptomic methodologies and analyses of both experimental and natural samples challenge the dogma of a restricted latency-associated transcription program. Instead, they portray the hallmark of HCMV latent infection as low-level expression of a broad spectrum of canonical viral lytic genes.
摘要
由于自然潜伏细胞的稀缺性和现有模型的复杂性，潜伏人巨细胞病毒（HCMV）的转录组一直极难定义。基因组时代为转录组谱分析提供了许多方法，这些方法为阐明这一具有挑战性的问题提供了巨大的潜力。最近两项应用不同转录组学方法并对实验和自然样本进行分析的研究结果，对受限的潜伏相关转录程序的教条提出了挑战。相反，它们描绘了 HCMV 潜伏感染的标志是广泛的典型病毒裂解基因的低水平表达。
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2
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Nature. 2019 Jul;571(7765):419-423. doi: 10.1038/s41586-019-1369-y. Epub 2019 Jul 10.
3
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5
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6
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