Department of Biomedical Informatics, Division of Health Sciences, Osaka University Graduate School of Medicine.
Department of Endocrinology and Diabetes in NTT West Osaka Hospital.
J Atheroscler Thromb. 2019 Oct 1;26(10):931-943. doi: 10.5551/jat.46797. Epub 2019 Mar 14.
In the pathogenesis of atherosclerosis, autoantibodies have two-facedness of progression and protection. Previous reports have indicated that low autoantibody levels against apolipoprotein B-100 (apo B-100) could increase the risk of atherosclerotic cardiovascular diseases (CVD) in healthy subjects. In this study, we investigated the relationship between circulating anti-apo B-100 autoantibodies and the clinical parameters in Japanese diabetic patients with or without CVD.
We measured the serum levels of anti-apo B-100 autoantibodies against native and malondialdehyde (MDA)-modified p45 or p210 epitopes, as well as anti-apo E autoantibodies, using enzyme-linked immunosorbent assay.
In patients with CVD, the circulating levels of IgG against native p45, MDA-modified p45, and MDA-modified p210 (IgG, IgG, and IgG) were significantly lower than those in patients without CVD, whereas no difference was observed in anti-apo E autoantibody levels. In addition, IgM, IgM, and IgG were negatively correlated with LDL-C levels, whereas IgG and IgG were positively correlated with HbA1c levels. No correlation was observed between autoantibody levels and diabetic microangiopathy. In the statin-treated subgroup, IgG and IgG were significantly lower in patients with CVD than in those without CVD.
Measurement of serum anti-apo B-100 autoantibodies can be useful for the evaluation of CVD risk in patients with diabetes receiving statin treatment.
在动脉粥样硬化的发病机制中，自身抗体具有促进进展和保护的两面性。先前的报告表明，针对载脂蛋白 B-100（apo B-100）的低自身抗体水平可能会增加健康受试者发生动脉粥样硬化性心血管疾病（CVD）的风险。在这项研究中，我们调查了循环抗 apo B-100 自身抗体与日本糖尿病患者伴或不伴 CVD 的临床参数之间的关系。
我们使用酶联免疫吸附试验测量了针对天然和丙二醛（MDA）修饰的 p45 或 p210 表位的抗 apo B-100 自身抗体以及抗 apo E 自身抗体的血清水平。
在 CVD 患者中，针对天然 p45、MDA 修饰的 p45 和 MDA 修饰的 p210（IgG、IgG 和 IgG）的 IgG 循环水平明显低于无 CVD 患者，而抗 apo E 自身抗体水平则无差异。此外，IgM、IgM 和 IgG 与 LDL-C 水平呈负相关，而 IgG 和 IgG 与 HbA1c 水平呈正相关。自身抗体水平与糖尿病微血管病变无相关性。在他汀类药物治疗亚组中，CVD 患者的 IgG 和 IgG 明显低于无 CVD 患者。
测量血清抗 apo B-100 自身抗体可用于评估接受他汀类药物治疗的糖尿病患者的 CVD 风险。
