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微小RNA-26b在宫颈癌中作为一种抑癌基因和预后因素发挥作用。

MicroRNA-26b acts as an antioncogene and prognostic factor in cervical cancer.

作者信息

Wang Lihong, Wang Wen, Wu Yuanyuan

机构信息

Department of Pathology, Shangluo Central Hospital, Shangluo, Shaanxi 726000, P.R. China.

出版信息

Oncol Lett. 2019 Mar;17(3):3418-3424. doi: 10.3892/ol.2019.9965. Epub 2019 Jan 24.

DOI:10.3892/ol.2019.9965
PMID:30867779
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6396109/
Abstract

Cervical cancer is the second most frequent malignant neoplasm in women all over the world. MicroRNA-26b (miR-26b) has been reported to be downregulated and play a great role in many malignancies, nevertheless, there are scarce studies on cervical cancer. The purpose of the present study was to detect how miR-26b is involved in cervical carcinoma. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was utilized to detect the expression levels of miR-26b and Jagged1 (JAG1) mRNA. Transwell assay was applied to calculate the cell migration and invasion capacity. Luciferase reporter assay was employed to determine JAG1 as a target of miR-26b. The results revealed that miR-26b is downregulated in cervical cancer tissues and cells compared with paracancerous tissues and normal cervical epithelial cells. The low expression of miR-26b in cervical cancer demonstrated that miR-26b inhibits cell migration and invasion, as measured by Transwell assay. JAG1 was verified to be a target of miR-26b and have a negative correlation with miR-26b, as detected by luciferase reporter assay. In addition, miR-26b was found to suppress cell migration and invasion via mediating JAG1 expression, which impact is partially reversed by JAG1. In conclusion, miR-26b suppresses cell migration and invasion of cervical cancer through directly targeting JAG1. It is suggested that miR-26b/JAG1 axis may present a new target for the treatment of cervical cancer.

摘要

宫颈癌是全球女性中第二常见的恶性肿瘤。据报道，微小RNA-26b（miR-26b）表达下调，且在许多恶性肿瘤中发挥重要作用，然而，关于宫颈癌的研究却很少。本研究的目的是检测miR-26b如何参与宫颈癌的发生发展。采用逆转录-定量聚合酶链反应（RT-qPCR）检测miR-26b和锯齿状蛋白1（JAG1）mRNA的表达水平。运用Transwell实验评估细胞迁移和侵袭能力。采用荧光素酶报告基因检测法确定JAG1为miR-26b的靶标。结果显示，与癌旁组织和正常宫颈上皮细胞相比，宫颈癌组织和细胞中miR-26b表达下调。通过Transwell实验检测发现，宫颈癌中miR-26b低表达表明其抑制细胞迁移和侵袭。荧光素酶报告基因检测法证实JAG1是miR-26b的靶标，且与miR-26b呈负相关。此外，发现miR-26b通过介导JAG1表达抑制细胞迁移和侵袭，而JAG1可部分逆转这种作用。总之，miR-26b通过直接靶向JAG1抑制宫颈癌细胞的迁移和侵袭。提示miR-26b/JAG1轴可能成为宫颈癌治疗的新靶点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e25/6396109/8bdaf16df05d/ol-17-03-3418-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e25/6396109/53cb42ec65d8/ol-17-03-3418-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e25/6396109/e7715fb2eb03/ol-17-03-3418-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e25/6396109/f172783aea3a/ol-17-03-3418-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e25/6396109/8bdaf16df05d/ol-17-03-3418-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e25/6396109/53cb42ec65d8/ol-17-03-3418-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e25/6396109/e7715fb2eb03/ol-17-03-3418-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e25/6396109/f172783aea3a/ol-17-03-3418-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9e25/6396109/8bdaf16df05d/ol-17-03-3418-g03.jpg

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