Zhao Xu, Hao Shuai, Wang Minqing, Xing Deguang, Wang Chengwei
Department of Neurosurgery, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
Department of Neurosurgery, People's Hospital of Juye County, Juye, Shandong 274900, P.R. China.
Oncol Lett. 2019 Mar;17(3):3511-3516. doi: 10.3892/ol.2019.9994. Epub 2019 Jan 31.
A large number of pseudogenes as well as long non-coding RNAs (lncRNAs) have been identified as important regulators in human tumors. However, the clinical role and potential functional effects of the double homeobox A pseudogene 8 (DUXAP8) in glioma remains unknown. In the present study, it was revealed that pseudogene DUXAP8 is significantly upregulated in glioma tissues, compared with adjacent normal tissues. Patients with increased DUXAP8 expression were associated with higher Karnofsky Performance Status, advanced World Health Organization grade, poor disease-free survival and overall survival rates of patients with glioma. Furthermore, in vitro assays, Cell-Counting Kit-8 cell viability and cell colony forming assays demonstrated that reduced DUXAP8 expression significantly suppressed proliferation capacity. Therefore, the results of the present study indicate that pseudogene DUXAP8 is an oncogenic lncRNA and may serve as a potentially prognostic biomarker and novel target of glioma treatment.
大量假基因以及长链非编码RNA（lncRNA）已被确定为人类肿瘤中的重要调节因子。然而，双同源盒A假基因8（DUXAP8）在胶质瘤中的临床作用和潜在功能影响仍不清楚。在本研究中，发现与相邻正常组织相比，假基因DUXAP8在胶质瘤组织中显著上调。DUXAP8表达增加的患者与较高的卡氏功能状态、世界卫生组织高级别、胶质瘤患者较差的无病生存率和总生存率相关。此外，在体外试验中，细胞计数试剂盒-8细胞活力和细胞集落形成试验表明，DUXAP8表达降低显著抑制增殖能力。因此，本研究结果表明，假基因DUXAP8是一种致癌lncRNA，可能作为胶质瘤治疗的潜在预后生物标志物和新靶点。
