敲低胶质瘤中假基因DUXAP8的表达可抑制肿瘤细胞增殖。
Knockdown of pseudogene DUXAP8 expression in glioma suppresses tumor cell proliferation.
作者信息
Zhao Xu, Hao Shuai, Wang Minqing, Xing Deguang, Wang Chengwei
机构信息
Department of Neurosurgery, The Second Hospital of Shandong University, Jinan, Shandong 250033, P.R. China.
Department of Neurosurgery, People's Hospital of Juye County, Juye, Shandong 274900, P.R. China.
出版信息
Oncol Lett. 2019 Mar;17(3):3511-3516. doi: 10.3892/ol.2019.9994. Epub 2019 Jan 31.
Abstract
A large number of pseudogenes as well as long non-coding RNAs (lncRNAs) have been identified as important regulators in human tumors. However, the clinical role and potential functional effects of the double homeobox A pseudogene 8 (DUXAP8) in glioma remains unknown. In the present study, it was revealed that pseudogene DUXAP8 is significantly upregulated in glioma tissues, compared with adjacent normal tissues. Patients with increased DUXAP8 expression were associated with higher Karnofsky Performance Status, advanced World Health Organization grade, poor disease-free survival and overall survival rates of patients with glioma. Furthermore, in vitro assays, Cell-Counting Kit-8 cell viability and cell colony forming assays demonstrated that reduced DUXAP8 expression significantly suppressed proliferation capacity. Therefore, the results of the present study indicate that pseudogene DUXAP8 is an oncogenic lncRNA and may serve as a potentially prognostic biomarker and novel target of glioma treatment.
摘要
大量假基因以及长链非编码RNA（lncRNA）已被确定为人类肿瘤中的重要调节因子。然而，双同源盒A假基因8（DUXAP8）在胶质瘤中的临床作用和潜在功能影响仍不清楚。在本研究中，发现与相邻正常组织相比，假基因DUXAP8在胶质瘤组织中显著上调。DUXAP8表达增加的患者与较高的卡氏功能状态、世界卫生组织高级别、胶质瘤患者较差的无病生存率和总生存率相关。此外，在体外试验中，细胞计数试剂盒-8细胞活力和细胞集落形成试验表明，DUXAP8表达降低显著抑制增殖能力。因此，本研究结果表明，假基因DUXAP8是一种致癌lncRNA，可能作为胶质瘤治疗的潜在预后生物标志物和新靶点。
相似文献
1
Knockdown of pseudogene DUXAP8 expression in glioma suppresses tumor cell proliferation.敲低胶质瘤中假基因DUXAP8的表达可抑制肿瘤细胞增殖。
Oncol Lett. 2019 Mar;17(3):3511-3516. doi: 10.3892/ol.2019.9994. Epub 2019 Jan 31.
2
DUXAP8, a pseudogene derived lncRNA, promotes growth of pancreatic carcinoma cells by epigenetically silencing CDKN1A and KLF2.DUXAP8，一个来源于假基因的长链非编码 RNA，通过表观遗传沉默 CDKN1A 和 KLF2 促进胰腺癌细胞的生长。
Cancer Commun (Lond). 2018 Oct 26;38(1):64. doi: 10.1186/s40880-018-0333-9.
3
The Pseudogene DUXAP8 Promotes Non-small-cell Lung Cancer Cell Proliferation and Invasion by Epigenetically Silencing EGR1 and RHOB.假基因DUXAP8通过表观遗传沉默EGR1和RHOB促进非小细胞肺癌细胞的增殖和侵袭。
Mol Ther. 2017 Mar 1;25(3):739-751. doi: 10.1016/j.ymthe.2016.12.018. Epub 2017 Jan 25.
4
The pseudogene derived long noncoding RNA DUXAP8 promotes gastric cancer cell proliferation and migration via epigenetically silencing PLEKHO1 expression.假基因衍生的长链非编码RNA DUXAP8通过表观遗传沉默PLEKHO1表达促进胃癌细胞增殖和迁移。
Oncotarget. 2016 Aug 5;8(32):52211-52224. doi: 10.18632/oncotarget.11075. eCollection 2017 Aug 8.
5
Pseudogene Promotes Cell Proliferation and Migration of Hepatocellular Carcinoma by Sponging MiR-490-5p to Induce BUB1 Expression.假基因通过吸附miR-490-5p诱导BUB1表达促进肝细胞癌的细胞增殖和迁移
Front Genet. 2020 Jul 22;11:666. doi: 10.3389/fgene.2020.00666. eCollection 2020.
6
Functions of lncRNA DUXAP8 in non-small cell lung cancer.长链非编码 RNA DUXAP8 在非小细胞肺癌中的功能。
Mol Biol Rep. 2022 Mar;49(3):2531-2542. doi: 10.1007/s11033-021-07066-6. Epub 2022 Jan 15.
7
Overexpressed pseudogenes, DUXAP8 and DUXAP9, promote growth of renal cell carcinoma and serve as unfavorable prognostic biomarkers.过表达的假基因DUXAP8和DUXAP9促进肾细胞癌的生长，并作为不良预后生物标志物。
Aging (Albany NY). 2019 Aug 13;11(15):5666-5688. doi: 10.18632/aging.102152.
8
Long Non-coding RNA Double Homeobox A Pseudogene 8: A Novel Oncogenic Propellant in Human Cancer.长链非编码RNA双同源盒A假基因8：人类癌症中的一种新型致癌推进剂。
Front Cell Dev Biol. 2021 Sep 23;9:709069. doi: 10.3389/fcell.2021.709069. eCollection 2021.
9
lncRNA DUXAP8 Facilitates Multiple Malignant Phenotypes and Resistance to PARP Inhibitor in HCC via Upregulating FOXM1.长链非编码RNA DUXAP8通过上调FOXM1促进肝癌的多种恶性表型及对PARP抑制剂的耐药性。
Mol Ther Oncolytics. 2020 Oct 22;19:308-322. doi: 10.1016/j.omto.2020.10.010. eCollection 2020 Dec 16.
10
The double homeobox a pseudogene 8 accelerates cell proliferation, migration, and invasion in colon cancer.双同源盒A假基因8促进结肠癌细胞的增殖、迁移和侵袭。
Bioengineered. 2022 Apr;13(4):8164-8173. doi: 10.1080/21655979.2022.2053802.
引用本文的文献
1
RARRES1 inhibits hepatocellular carcinoma progression and increases its sensitivity to lenvatinib through interaction with SPINK2.RARRES1 通过与 SPINK2 相互作用抑制肝细胞癌进展并增加其对仑伐替尼的敏感性。
Biol Direct. 2024 Feb 23;19(1):15. doi: 10.1186/s13062-024-00459-0.
2
Pseudogene MSTO2P Interacts with miR-128-3p to Regulate Coptisine Sensitivity of Non-Small-Cell Lung Cancer (NSCLC) through TGF- Signaling and VEGFC.假基因MSTO2P与miR-128-3p相互作用，通过TGF信号和VEGFC调节非小细胞肺癌（NSCLC）对黄连碱的敏感性。
J Oncol. 2022 Jun 26;2022:9864411. doi: 10.1155/2022/9864411. eCollection 2022.
3
The double homeobox a pseudogene 8 accelerates cell proliferation, migration, and invasion in colon cancer.双同源盒A假基因8促进结肠癌细胞的增殖、迁移和侵袭。
Bioengineered. 2022 Apr;13(4):8164-8173. doi: 10.1080/21655979.2022.2053802.
4
m6A Modification-Mediated Regulation of Malignant Phenotype and Chemotherapy Resistance of Hepatocellular Carcinoma Through miR-584-5p/MAPK1/ERK Pathway Axis.通过miR-584-5p/MAPK1/ERK信号通路轴，m6A修饰介导的肝细胞癌恶性表型和化疗耐药性调控
Front Cell Dev Biol. 2021 Dec 9;9:783385. doi: 10.3389/fcell.2021.783385. eCollection 2021.
5
Pseudogene RPL32P3 regulates the blood-tumor barrier permeability via the YBX2/HNF4G axis.假基因RPL32P3通过YBX2/HNF4G轴调节血肿瘤屏障通透性。
Cell Death Discov. 2021 Nov 24;7(1):367. doi: 10.1038/s41420-021-00758-9.
6
Long non-coding RNA DUXAP8 promotes tumorigenesis by regulating IGF1R via miR-9-3p in hepatocellular carcinoma.长链非编码RNA DUXAP8通过在肝细胞癌中经由miR-9-3p调控IGF1R来促进肿瘤发生。
Exp Ther Med. 2021 Jul;22(1):755. doi: 10.3892/etm.2021.10187. Epub 2021 May 12.
7
YY1-inudced activation of lncRNA DUXAP8 promotes proliferation and suppresses apoptosis of triple negative breast cancer cells through upregulating SAPCD2.YY1 诱导的 lncRNA DUXAP8 通过上调 SAPCD2 促进三阴性乳腺癌细胞的增殖并抑制凋亡。
Cancer Biol Ther. 2021 Mar 4;22(3):216-224. doi: 10.1080/15384047.2021.1881201. Epub 2021 Mar 8.
8
Silencing lncRNA DUXAP8 inhibits lung adenocarcinoma progression by targeting miR-26b-5p.沉默 lncRNA DUXAP8 通过靶向 miR-26b-5p 抑制肺腺癌进展。
Biosci Rep. 2021 Jan 29;41(1). doi: 10.1042/BSR20200884.
9
Long Non-Coding RNA DUXAP8 Facilitates Cell Viability, Migration, and Glycolysis in Non-Small-Cell Lung Cancer via Regulating HK2 and LDHA by Inhibition of miR-409-3p.长链非编码RNA DUXAP8通过抑制miR-409-3p调控己糖激酶2（HK2）和乳酸脱氢酶A（LDHA）促进非小细胞肺癌的细胞活力、迁移及糖酵解。
Onco Targets Ther. 2020 Jul 22;13:7111-7123. doi: 10.2147/OTT.S243542. eCollection 2020.
10
Upregulation of COL8A1 indicates poor prognosis across human cancer types and promotes the proliferation of gastric cancer cells.COL8A1的上调表明在多种人类癌症类型中预后不良，并促进胃癌细胞的增殖。
Oncol Lett. 2020 Oct;20(4):34. doi: 10.3892/ol.2020.11895. Epub 2020 Jul 21.
本文引用的文献
1
A genome-wide comprehensively analyses of long noncoding RNA profiling and metastasis associated lncRNAs in renal cell carcinoma.肾细胞癌中长链非编码RNA谱及转移相关长链非编码RNA的全基因组综合分析。
Oncotarget. 2017 Sep 23;8(50):87773-87781. doi: 10.18632/oncotarget.21206. eCollection 2017 Oct 20.
2
Identification of potential cancer-related pseudogenes in lung adenocarcinoma based on ceRNA hypothesis.基于ceRNA假说鉴定肺腺癌中潜在的癌症相关假基因
Oncotarget. 2017 Aug 4;8(35):59036-59047. doi: 10.18632/oncotarget.19933. eCollection 2017 Aug 29.
3
The pseudogene derived long noncoding RNA DUXAP8 promotes gastric cancer cell proliferation and migration via epigenetically silencing PLEKHO1 expression.假基因衍生的长链非编码RNA DUXAP8通过表观遗传沉默PLEKHO1表达促进胃癌细胞增殖和迁移。
Oncotarget. 2016 Aug 5;8(32):52211-52224. doi: 10.18632/oncotarget.11075. eCollection 2017 Aug 8.
4
The Pseudogene DUXAP8 Promotes Non-small-cell Lung Cancer Cell Proliferation and Invasion by Epigenetically Silencing EGR1 and RHOB.假基因DUXAP8通过表观遗传沉默EGR1和RHOB促进非小细胞肺癌细胞的增殖和侵袭。
Mol Ther. 2017 Mar 1;25(3):739-751. doi: 10.1016/j.ymthe.2016.12.018. Epub 2017 Jan 25.
5
Glioma: experimental models and reality.胶质瘤：实验模型与现实。
Acta Neuropathol. 2017 Feb;133(2):263-282. doi: 10.1007/s00401-017-1671-4. Epub 2017 Jan 10.
6
A systematic review and meta-analysis of gene therapy in animal models of cerebral glioma: why did promise not translate to human therapy?脑胶质瘤动物模型中基因治疗的系统评价与荟萃分析：为何前景未能转化为人类治疗？
Evid Based Preclin Med. 2014 Dec;1(1):e00006. doi: 10.1002/ebm2.6. Epub 2015 Jan 20.
7
Inhibitory effect of RNA-mediated knockdown of zinc finger protein 91 pseudogene on pancreatic cancer cell growth and invasion.RNA介导的锌指蛋白91假基因敲低对胰腺癌细胞生长和侵袭的抑制作用
Oncol Lett. 2016 Aug;12(2):1343-1348. doi: 10.3892/ol.2016.4794. Epub 2016 Jun 28.
8
Increased expression of SUMO1P3 predicts poor prognosis and promotes tumor growth and metastasis in bladder cancer.SUMO1P3表达增加预示膀胱癌预后不良，并促进肿瘤生长和转移。
Oncotarget. 2016 Mar 29;7(13):16038-48. doi: 10.18632/oncotarget.6946.
9
Functional roles of pseudogenes in cancers.假基因在癌症中的功能作用。
Yi Chuan. 2015 Jan;37(1):8-16. doi: 10.16288/j.yczz.2015.01.002.
10
Pseudogene in cancer: real functions and promising signature.癌症中的假基因：真实功能与前景广阔的标志物
J Med Genet. 2015 Jan;52(1):17-24. doi: 10.1136/jmedgenet-2014-102785. Epub 2014 Nov 12.
Zotero 插件