Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia.
Haql College, University of Tabuk, Tabuk 71491, Saudi Arabia.
Biomed Res Int. 2019 Feb 5;2019:7064073. doi: 10.1155/2019/7064073. eCollection 2019.
The present study has synthesized poly(4,4'-cyclohexylidene bisphenol oxalate) by the condensation of oxalyl chloride with 4,4'-cyclohexylidene bisphenol, where its efficacy was tested for the solid-phase extraction of DNA. The synthesized polymer in the form of a white powder was characterized by FTIR, TGA-DTG, SEM, and BET analysis. The study utilized solid-phase application of the resulting polymer to extract DNA. The analysis of results provided the information that the extraction efficiency is a strong dependent of polymer amount and binding buffer type. Among the three types of buffers tested, the GuHCl buffer produced the most satisfactory results in terms of yield and efficiency of extraction. Moreover, the absorbance ratio of A260/A280 in all of the samples varied from 1.682 to 1.491, thereby confirming the capability of poly(4,4'-cyclohexylidene bisphenol oxalate) to elute pure DNA. The results demonstrated an increased DNA binding capacity with respect to increased percentage of the polymer. The study has concluded that poly(bisphenol Z oxalate) can be applied as one of the potential candidates for the high efficiency extraction of DNA by means of a simple, cost-effective, and environmentally friendly approach compared to the other traditional solid-phase methods.
本研究通过草酰氯与 4,4'-环己基二苯酚的缩合反应合成了聚（4,4'-环己基二苯酚草酸酯），并测试了其对 DNA 的固相萃取效果。合成的聚合物呈白色粉末状，通过 FTIR、TGA-DTG、SEM 和 BET 分析进行了表征。研究利用所得聚合物的固相应用来提取 DNA。结果分析提供的信息表明，萃取效率强烈依赖于聚合物的用量和结合缓冲液的类型。在所测试的三种缓冲液中，盐酸胍缓冲液在产率和提取效率方面产生了最令人满意的结果。此外，所有样品的吸光度比 A260/A280 均从 1.682 变化到 1.491，从而证实了聚（4,4'-环己基二苯酚草酸酯）洗脱纯 DNA 的能力。结果表明，随着聚合物百分比的增加，DNA 结合能力增加。该研究得出结论，与其他传统固相方法相比，聚（双酚 Z 草酸酯）可以作为高效提取 DNA 的潜在候选物之一，通过简单、经济高效和环保的方法应用。
