Doctorado en Ciencias Biomédicas, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
Instituto de Investigación en Odontología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Guadalajara, Jalisco, Mexico.
Biomed Res Int. 2019 Feb 5;2019:7850392. doi: 10.1155/2019/7850392. eCollection 2019.
Chronic periodontitis (CP) is an infection that affects the teeth supporting structure. Macrophage migration inhibitory factor (MIF) is an important effector cytokine of the innate immune system. Due to its functional characteristics, MIF may be involved in the immunopathology of CP. The aim of the present study was to evaluate MIF levels in gingival crevicular fluid (GCF), saliva, and serum of CP patients. A cross-sectional study was conducted on 60 subjects divided into two groups: subjects with CP (n= 30) and periodontally healthy subjects without CP (n=30). MIF was quantified in GCF, saliva, and serum of all participants by enzyme-linked immunosorbent assay. MIF concentrations were higher in GCF, saliva, and serum in the group with CP compared with the group without CP and a higher MIF concentration was observed in GCF (p=0.001) and saliva (p=0.009) in the group with CP. MIF intragroup comparisons between fluids demonstrated significant high levels of MIF in saliva compared with GCF and serum in both study groups (p<0.05). A positive correlation was found between clinical signs and MIF concentration in GCF (p<0.05). There is an association between the MIF and the clinical signs of the disease. Therefore, MIF could have an important role in the pathology and progression of CP.
慢性牙周炎（CP）是一种影响牙齿支持结构的感染。巨噬细胞移动抑制因子（MIF）是先天免疫系统的重要效应细胞因子。由于其功能特性，MIF 可能参与 CP 的免疫病理学。本研究旨在评估 CP 患者龈沟液（GCF）、唾液和血清中的 MIF 水平。对 60 名受试者进行了横断面研究，分为两组：CP 患者（n=30）和无 CP 的牙周健康受试者（n=30）。通过酶联免疫吸附试验定量测定所有参与者的 GCF、唾液和血清中的 MIF。与无 CP 组相比，CP 组的 GCF、唾液和血清中的 MIF 浓度更高，CP 组的 GCF（p=0.001）和唾液（p=0.009）中的 MIF 浓度更高。两组间的 MIF 组内比较显示，唾液中的 MIF 水平明显高于 GCF 和血清（p<0.05）。在 GCF 中，临床体征与 MIF 浓度之间存在正相关（p<0.05）。MIF 与疾病的临床体征之间存在关联。因此，MIF 可能在 CP 的发病机制和进展中发挥重要作用。
