Department of Periodontology, Service of Odontology, AP-HM, UFR of Odontology, Aix-Marseille University, Marseille, France.
AP-HM, IHU-Méditerranée Infection, Institut de Recherche pour le Développement, Institut Hospitalo-Universitaire Méditerranée Infection, MEPHI, Aix Marseille University, Marseille, France.
PLoS One. 2020 Mar 13;15(3):e0230334. doi: 10.1371/journal.pone.0230334. eCollection 2020.
The aim of the present study was to assess the feasibility and diagnostic contribution of protein profiling using MALDI-TOF mass spectrometry applied to saliva, gingival crevicular fluid (GCF) and dental plaque from periodontitis and healthy subjects. We hypothesized that rapid routine and blinded MALDI-TOF analysis could accurately classify these three types of samples according to periodontal state.
Unstimulated saliva, GCF and dental plaque, collected from periodontitis subjects and healthy controls, were analyzed by MALDI-TOF MS. Based on the differentially expressed peaks between the two groups, diagnostic decision trees were built for each sample.
Among 141 patients (67 periodontitis and 74 healthy controls), the decision trees diagnosed periodontitis with a sensitivity = 70.3% (± 0.211) and a specificity = 77.8% (± 0.165) for saliva, a sensitivity = 79.6% (± 0.188) and a specificity = 75.7% (± 0.195) for GCF, and a sensitivity = 72.1% (± 0.202) and a specificity = 72.2% (± 0.195) for dental plaque. The sensitivity and specificity of the tests were improved to 100% (CI 95% = [0.91;1]) and 100% (CI 95% = [0.92;1]), respectively, when two samples were tested.
We developed, for the first time, diagnostic tests based on protein profiles of saliva, GCF and dental plaque between periodontitis patients and healthy subjects. When at least 2 of these samples were tested, the best results were obtained.
本研究旨在评估 MALDI-TOF 质谱法在唾液、龈沟液（GCF）和牙周炎及健康受试者牙菌斑中的蛋白质谱分析的可行性和诊断贡献。我们假设快速常规和盲法 MALDI-TOF 分析能够根据牙周状况准确地对这三种类型的样本进行分类。
使用 MALDI-TOF MS 分析来自牙周炎患者和健康对照组的未刺激唾液、GCF 和牙菌斑。基于两组之间差异表达的峰，为每种样本构建诊断决策树。
在 141 名患者（67 名牙周炎患者和 74 名健康对照组）中，决策树诊断牙周炎的唾液敏感性为 70.3%（±0.211），特异性为 77.8%（±0.165），GCF 的敏感性为 79.6%（±0.188），特异性为 75.7%（±0.195），牙菌斑的敏感性为 72.1%（±0.202），特异性为 72.2%（±0.195）。当测试两种样本时，测试的敏感性和特异性提高到 100%（95%CI[0.91;1]）和 100%（95%CI[0.92;1]）。
我们首次开发了基于牙周炎患者和健康受试者之间唾液、GCF 和牙菌斑的蛋白质谱分析的诊断测试。当至少测试两种样本时，可获得最佳结果。
