Berthier F, Renaud M, Alziari S, Durand R
Nucleic Acids Res. 1986 Jun 11;14(11):4519-33. doi: 10.1093/nar/14.11.4519.
Drosophila melanogaster mitochondrial DNA (mtDNA) is closely related to the mammalian and amphibian mtDNA except for gene organization. In Drosophila, genes are distributed in clusters alternatively coded on each strand. Besides the eleven major foreseeable transcripts previously described (MERTEN and PARDUE, 1981, J. Mol. Biol., 153, 1-21), we have characterized two poly A+ transcripts, one major and one minor which could correspond respectively to the ND3 and ND6 reading frames, and 27 poly A+ minor transcripts (0.2 to greater than 3.2 kb) which are distributed along the mtDNA except in the rRNAs, ND 1 and A+ T rich regions. The mapping and length of 25 of these transcripts strongly suggest a precursor role. They would be processed at the level of tRNA or tRNA-like sequences. Most of them are transcribed from the template strand of each gene cluster and their distribution is in agreement with the hypothesis of several transcription origins and terminations located near the extremities of each gene cluster. Quantitatively our results show a large variation in each presumptive mature transcript compared to the other, even in a given gene cluster, suggesting a specific degradation of some of the mature transcripts.
除基因组织外，果蝇线粒体DNA（mtDNA）与哺乳动物和两栖动物的mtDNA密切相关。在果蝇中，基因成簇分布，每条链上交替编码。除了先前描述的11种主要可预见转录本（MERTEN和PARDUE，1981，《分子生物学杂志》，153，1 - 21）外，我们还鉴定了两种聚腺苷酸加尾转录本，一种主要的和一种次要的，它们可能分别对应于ND3和ND6阅读框，以及27种聚腺苷酸加尾次要转录本（0.2至大于3.2 kb），它们分布在线粒体DNA上，除了rRNA、ND1和富含A + T的区域。其中25种转录本的定位和长度强烈表明其具有前体作用。它们将在tRNA或类tRNA序列水平上进行加工。它们中的大多数从每个基因簇的模板链转录而来，其分布与每个基因簇末端附近存在多个转录起始和终止的假设一致。从数量上看，我们的结果表明，与其他转录本相比，即使在给定的基因簇中，每个假定的成熟转录本也存在很大差异，这表明一些成熟转录本存在特异性降解。
