Batuecas B, Garesse R, Calleja M, Valverde J R, Marco R
Departamento de Bioquímica, Facultad de Medicina, Universidad Autónoma de Madrid, Spain.
Nucleic Acids Res. 1988 Jul 25;16(14A):6515-29. doi: 10.1093/nar/16.14.6515.
To extend to the crustacean class the information concerning the genomic organization of the mitochondrial DNA (mtDNA) a double strategy has been used: Southern blot analysis with cloned Drosophila mtDNA probes and sequence comparison to the Drosophila mtDNA of the sequenced termini of different subclones along the Artemia mitochondrial genome, probably the smallest mtDNA studied at this level to date. These approaches have allowed us to localize the 16S rRNA gene, two tRNA genes and eleven protein genes. The genome organization is surprisingly similar to the Drosophila mtDNA, with the 16S rRNA and the protein genes located in the same positions and orientations as their Drosophila counterparts. The only changes detected are at the level of tRNA genes, although the position and orientation of some of these are also conserved. These results contrast with the important rearrangements detected among other invertebrates mtDNAs and suggest that the genome organization of the mitochondrial DNA may be more conserved in the arthropods than in other invertebrate phyla.
为了将有关线粒体DNA（mtDNA）基因组组织的信息扩展到甲壳纲动物，采用了双重策略：使用克隆的果蝇mtDNA探针进行Southern印迹分析，并将卤虫线粒体基因组中不同亚克隆的测序末端与果蝇mtDNA进行序列比较，卤虫线粒体基因组可能是迄今为止在这个层面上研究的最小的mtDNA。这些方法使我们能够定位16S rRNA基因、两个tRNA基因和11个蛋白质基因。基因组组织与果蝇mtDNA惊人地相似，16S rRNA和蛋白质基因的位置和方向与其果蝇对应物相同。检测到的唯一变化是在tRNA基因层面，尽管其中一些基因的位置和方向也保持保守。这些结果与在其他无脊椎动物mtDNA中检测到的重要重排形成对比，并表明线粒体DNA的基因组组织在节肢动物中可能比在其他无脊椎动物门中更保守。
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