人类CK基因片段与κ缺失元件紧密相连。
The human CK gene segment and the kappa deleting element are closely linked.
作者信息
Klobeck H G, Zachau H G
出版信息
Nucleic Acids Res. 1986 Jun 11;14(11):4591-603. doi: 10.1093/nar/14.11.4591.
Abstract
The element which mediates the deletion of the CK gene segment (abbreviations ref. 1) in human lambda light chain producing B-cells was found to be located 24 kb downstream of CK. The kappa deleting element is flanked by hepta- and nonanucleotide recognition sequences similar to the ones adjacent to the JK gene segments. Complementary recognition sequences with a 30 bp spacer were found in the JK-CK intron. For the first time the two partners of a recombination event in a mammalian immunoglobulin gene system have been linked on a physical map. The orientation of the recombination signals of the intron and the Kde sequences allows a joining by a simple deletion mechanism. Similarities and possible differences to the mechanism of V-J joining are discussed.
摘要
在产生人λ轻链的B细胞中，介导CK基因片段（参考文献1中的缩写）缺失的元件位于CK下游24 kb处。κ链缺失元件两侧是七核苷酸和九核苷酸识别序列，类似于与JK基因片段相邻的序列。在JK-CK内含子中发现了带有30 bp间隔区的互补识别序列。在哺乳动物免疫球蛋白基因系统中，重组事件的两个参与元件首次在物理图谱上被关联起来。内含子和Kde序列的重组信号方向允许通过简单的缺失机制进行连接。文中还讨论了与V-J连接机制的异同。
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