Klobeck H G, Zimmer F J, Combriato G, Zachau H G
Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie, Universität München, FRG.
Nucleic Acids Res. 1987 Dec 10;15(23):9655-65. doi: 10.1093/nar/15.23.9655.
The linking of the human VK and JKCK gene regions (abbreviations in ref. 1) by chromosomal walking is reported. Hybridization experiments with the DNA of a somatic cell hybrid containing the region between JKCK and the telomer show that none of the major VK gene clusters is located downstream of CK. The distance between the VK and JK genes was found to be 23 kb. The JK proximal VK gene is the B3 gene which is the only representative of subgroup IV in the genome. This gene and the neighbouring B2 gene (accompanying paper) are arranged in opposite orientation to JKCK and can therefore rearrange only by an inversion mechanism. This finding is used, together with previous data, to delineate the rearrangement processes in the Burkitt lymphoma derived cell line BL21 as comprising an inversion in the first and a deletion in the second step.
本文报道了通过染色体步移法将人类VK和JKCK基因区域（参考文献1中的缩写）连接起来的研究。对含有JKCK和端粒之间区域的体细胞杂种DNA进行杂交实验，结果表明，主要的VK基因簇均不在CK下游。发现VK和JK基因之间的距离为23 kb。JK近端的VK基因是B3基因，它是基因组中IV亚组的唯一代表。该基因和相邻的B2基因（随附论文）与JKCK呈相反方向排列，因此只能通过倒位机制进行重排。这一发现与先前的数据一起，用于描述伯基特淋巴瘤衍生细胞系BL21中的重排过程，该过程包括第一步的倒位和第二步的缺失。
