Medeiros Nayara I, Gomes Juliana A S
Cellular and Molecular Immunology, René Rachou Institute, Oswaldo Cruz Foundation, Belo Horizonte, Minas Gerais, Brazil.
Laboratory of Cell-Cell Interactions, Department of Morphology, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Methods Mol Biol. 2019;1955:309-314. doi: 10.1007/978-1-4939-9148-8_23.
Cytometric bead array (CBA) is a flow cytometry application that allows users to quantify multiple proteins simultaneously. Compared to other quantifier assays, as enzyme-linked immunosorbent assay (ELISA) and Western blot, CBA significantly reduces sample requirements and time to results. This technology allows for the design and creation of assays to measure a variety of analytes including inflammatory mediators, chemokines, immunoglobulin isotypes, intracellular signaling molecules, apoptotic mediators, adhesion molecules, and antibodies. Here we describe CBA steps to measure soluble cytokine levels in body fluids of infected patients by the protozoan Trypanosoma cruzi, morbidity known as Chagas disease.
细胞计数珠阵列（CBA）是一种流式细胞术应用，可让用户同时对多种蛋白质进行定量。与其他定量分析方法如酶联免疫吸附测定（ELISA）和蛋白质印迹法相比，CBA显著减少了样本需求并缩短了出结果的时间。该技术可用于设计和创建检测多种分析物的分析方法，包括炎症介质、趋化因子、免疫球蛋白亚型、细胞内信号分子、凋亡介质、黏附分子和抗体。在此，我们描述了通过原生动物克氏锥虫（Trypanosoma cruzi）（一种导致恰加斯病的寄生虫）感染患者的体液中可溶性细胞因子水平的CBA检测步骤。
